Difference between revisions of "Part:BBa K1350003:Experience"
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This is a part of Alkaline cellulase.We obtain the gene through the PCR method and recombine it in plasmid pET-28a which has strong expression ability, and we transform the recombinant plasmid in a strong protein expression system—— BL21 Escherichia coli ,then put in iptg artificially to induce alkaline cellulase. | This is a part of Alkaline cellulase.We obtain the gene through the PCR method and recombine it in plasmid pET-28a which has strong expression ability, and we transform the recombinant plasmid in a strong protein expression system—— BL21 Escherichia coli ,then put in iptg artificially to induce alkaline cellulase. | ||
| − | + | ===Application in deinking production=== | |
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=Mechanical synergy theory= | =Mechanical synergy theory= | ||
[[File:Mechanism.jpg]] | [[File:Mechanism.jpg]] | ||
Revision as of 18:41, 16 October 2014
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Applications of BBa_K1350003
This is a part of Alkaline cellulase.We obtain the gene through the PCR method and recombine it in plasmid pET-28a which has strong expression ability, and we transform the recombinant plasmid in a strong protein expression system—— BL21 Escherichia coli ,then put in iptg artificially to induce alkaline cellulase.
Application in deinking production
Mechanical synergy theory
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