Difference between revisions of "Part:BBa K1463600"
| Line 9: | Line 9: | ||
For more information on the biobrick and methods used go to http://2014.igem.org/wiki/index.php?title=Team:Glasgow/Project/Mobility_Proteins#motA | For more information on the biobrick and methods used go to http://2014.igem.org/wiki/index.php?title=Team:Glasgow/Project/Mobility_Proteins#motA | ||
| + | |||
| + | <html><!--- Please copy this table containing parameters for BBa_ at the end of the parametrs section ahead of the references. ---><style type="text/css">table#AutoAnnotator {border:1px solid black; width:100%; border-collapse:collapse;} th#AutoAnnotatorHeader { border:1px solid black; width:100%; background-color: rgb(221, 221, 221);} td.AutoAnnotator1col { width:100%; border:1px solid black; } span.AutoAnnotatorSequence { font-family:'Courier New', Arial; } td.AutoAnnotatorSeqNum { text-align:right; width:2%; } td.AutoAnnotatorSeqSeq { width:98% } td.AutoAnnotatorSeqFeat1 { width:3% } td.AutoAnnotatorSeqFeat2a { width:27% } td.AutoAnnotatorSeqFeat2b { width:97% } td.AutoAnnotatorSeqFeat3 { width:70% } table.AutoAnnotatorNoBorder { border:0px; width:100%; border-collapse:collapse; } table.AutoAnnotatorWithBorder { border:1px solid black; width:100%; border-collapse:collapse; } td.AutoAnnotatorOuterAmino { border:0px solid black; width:20% } td.AutoAnnotatorInnerAmino { border:1px solid black; width:50% } td.AutoAnnotatorAminoCountingOuter { border:1px solid black; width:40%; } td.AutoAnnotatorBiochemParOuter { border:1px solid black; width:60%; } td.AutoAnnotatorAminoCountingInner1 { width: 7.5% } td.AutoAnnotatorAminoCountingInner2 { width:62.5% } td.AutoAnnotatorAminoCountingInner3 { width:30% } td.AutoAnnotatorBiochemParInner1 { width: 5% } td.AutoAnnotatorBiochemParInner2 { width:55% } td.AutoAnnotatorBiochemParInner3 { width:40% } td.AutoAnnotatorCodonUsage1 { width: 3% } td.AutoAnnotatorCodonUsage2 { width:14.2% } td.AutoAnnotatorCodonUsage3 { width:13.8% } td.AutoAnnotatorAlignment1 { width: 3% } td.AutoAnnotatorAlignment2 { width: 10% } td.AutoAnnotatorAlignment3 { width: 87% } td.AutoAnnotatorLocalizationOuter {border:1px solid black; width:40%} td.AutoAnnotatorGOOuter {border:1px solid black; width:60%} td.AutoAnnotatorLocalization1 { width: 7.5% } td.AutoAnnotatorLocalization2 { width: 22.5% } td.AutoAnnotatorLocalization3 { width: 70% } td.AutoAnnotatorGO1 { width: 5% } td.AutoAnnotatorGO2 { width: 35% } td.AutoAnnotatorGO3 { width: 60% } td.AutoAnnotatorPredFeat1 { width:3% } td.AutoAnnotatorPredFeat2a { width:27% } td.AutoAnnotatorPredFeat3 { width:70% } div.AutoAnnotator_trans { position:absolute; background:rgb(11,140,143); background-color:rgba(11,140,143, 0.8); height:5px; top:100px; } div.AutoAnnotator_sec_helix { position:absolute; background:rgb(102,0,102); background-color:rgba(102,0,102, 0.8); height:5px; top:110px; } div.AutoAnnotator_sec_strand { position:absolute; background:rgb(245,170,26); background-color:rgba(245,170,26, 1); height:5px; top:110px; } div.AutoAnnotator_acc_buried { position:absolute; background:rgb(89,168,15); background-color:rgba(89,168,15, 0.8); height:5px; top:120px; } div.AutoAnnotator_acc_exposed { position:absolute; background:rgb(0, 0, 255); background-color:rgba(0, 0, 255, 0.8); height:5px; top:120px; } div.AutoAnnotator_dis { position:absolute; text-align:center; font-family:Arial,Helvetica,sans-serif; background:rgb(255, 200, 0); background-color:rgba(255, 200, 0, 1); height:16px; width:16px; top:80px; border-radius:50%; } </style><div id='AutoAnnotator_container_1413480945739'><table id="AutoAnnotator"><tr><!-- Time stamp in ms since 1/1/1970 1413480945739 --><th id="AutoAnnotatorHeader" colspan="2">Protein data table for BioBrick <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K1463600<!------------------------Enter BioBrick number here------------------------>">BBa_K1463600<!------------------------Enter BioBrick number here------------------------></a> automatically created by the <a href="http://2013.igem.org/Team:TU-Munich/Results/AutoAnnotator">BioBrick-AutoAnnotator</a> version 1.0</th></tr><tr><td class="AutoAnnotator1col" colspan="2"><strong>Nucleotide sequence</strong> in <strong>RFC 10</strong>: (underlined part encodes the protein)<br><span class="AutoAnnotatorSequence"> <u>ATGGCACAA ... CTCCAGGGT</u>TAA</span><br> <strong>ORF</strong> from nucleotide position 1 to 1494 (excluding stop-codon)</td></tr><tr><td class="AutoAnnotator1col" colspan="2"><strong>Amino acid sequence:</strong> (RFC 25 scars in shown in bold, other sequence features underlined; both given below)<br><span class="AutoAnnotatorSequence"><table class="AutoAnnotatorNoBorder"><tr><td class="AutoAnnotatorSeqNum">1 <br>101 <br>201 <br>301 <br>401 </td><td class="AutoAnnotatorSeqSeq">MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDDAAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRVRELTVQAT<br>TGTNSESDLSSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKNGSMKIQVGANDNQTITIDLKQIDAKTLGLDGFSVKNNDTVTTSAPVTAFGATTTNNIKL<br>TGITLSTEAATDTGGTNPASIEGVYTDNGNDYYAKITGGDNDGKYYAVTVANDGTVTMATGATANATVTDANTTKATTITSGGTPVQIDNTAGSATANLG<br>AVSLVKLQDSKGNDTDTYALKDTNGNLYAADVNETTGAVSVKTITYTDSSGAASSPTAVKLGGDDGKTEVVDIDGKTYDSADLNGGNLQTGLTAGGEALT<br>AVANGKTTDPLKALDDAIASVDKFRSSLGAVQNRLDSAVTNLNNTTTNLSEAQSRIQDADYATEVSNMSKAQIIQQAGNSVLAKANQVPQQVLSLLQG*</td></tr></table></span></td></tr><tr><td class="AutoAnnotator1col" colspan="2"><strong>Sequence features:</strong> (with their position in the amino acid sequence, see the <a href="http://2013.igem.org/Team:TU-Munich/Results/Software/FeatureList">list of supported features</a>)<table class="AutoAnnotatorNoBorder"><tr><td class="AutoAnnotatorSeqFeat1"></td><td class="AutoAnnotatorSeqFeat2b">None of the supported features appeared in the sequence</td></tr></table></td></tr><tr><td class="AutoAnnotator1col" colspan="2"><strong>Amino acid composition:</strong><table class="AutoAnnotatorNoBorder"><tr><td class="AutoAnnotatorOuterAmino"><table class="AutoAnnotatorWithBorder"><tr><td class="AutoAnnotatorInnerAmino">Ala (A)</td><td class="AutoAnnotatorInnerAmino">59 (11.8%)</td></tr><tr><td class="AutoAnnotatorInnerAmino">Arg (R)</td><td class="AutoAnnotatorInnerAmino">11 (2.2%)</td></tr><tr><td class="AutoAnnotatorInnerAmino">Asn (N)</td><td class="AutoAnnotatorInnerAmino">48 (9.6%)</td></tr><tr><td class="AutoAnnotatorInnerAmino">Asp (D)</td><td class="AutoAnnotatorInnerAmino">39 (7.8%)</td></tr></table></td><td class="AutoAnnotatorOuterAmino"><table class="AutoAnnotatorWithBorder"><tr><td class="AutoAnnotatorInnerAmino">Cys (C)</td><td class="AutoAnnotatorInnerAmino">0 (0.0%)</td></tr><tr><td class="AutoAnnotatorInnerAmino">Gln (Q)</td><td class="AutoAnnotatorInnerAmino">27 (5.4%)</td></tr><tr><td class="AutoAnnotatorInnerAmino">Glu (E)</td><td class="AutoAnnotatorInnerAmino">14 (2.8%)</td></tr><tr><td class="AutoAnnotatorInnerAmino">Gly (G)</td><td class="AutoAnnotatorInnerAmino">44 (8.8%)</td></tr></table></td><td class="AutoAnnotatorOuterAmino"><table class="AutoAnnotatorWithBorder"><tr><td class="AutoAnnotatorInnerAmino">His (H)</td><td class="AutoAnnotatorInnerAmino">0 (0.0%)</td></tr><tr><td class="AutoAnnotatorInnerAmino">Ile (I)</td><td class="AutoAnnotatorInnerAmino">28 (5.6%)</td></tr><tr><td class="AutoAnnotatorInnerAmino">Leu (L)</td><td class="AutoAnnotatorInnerAmino">37 (7.4%)</td></tr><tr><td class="AutoAnnotatorInnerAmino">Lys (K)</td><td class="AutoAnnotatorInnerAmino">25 (5.0%)</td></tr></table></td><td class="AutoAnnotatorOuterAmino"><table class="AutoAnnotatorWithBorder"><tr><td class="AutoAnnotatorInnerAmino">Met (M)</td><td class="AutoAnnotatorInnerAmino">4 (0.8%)</td></tr><tr><td class="AutoAnnotatorInnerAmino">Phe (F)</td><td class="AutoAnnotatorInnerAmino">5 (1.0%)</td></tr><tr><td class="AutoAnnotatorInnerAmino">Pro (P)</td><td class="AutoAnnotatorInnerAmino">6 (1.2%)</td></tr><tr><td class="AutoAnnotatorInnerAmino">Ser (S)</td><td class="AutoAnnotatorInnerAmino">43 (8.6%)</td></tr></table></td><td class="AutoAnnotatorOuterAmino"><table class="AutoAnnotatorWithBorder"><tr><td class="AutoAnnotatorInnerAmino">Thr (T)</td><td class="AutoAnnotatorInnerAmino">65 (13.1%)</td></tr><tr><td class="AutoAnnotatorInnerAmino">Trp (W)</td><td class="AutoAnnotatorInnerAmino">0 (0.0%)</td></tr><tr><td class="AutoAnnotatorInnerAmino">Tyr (Y)</td><td class="AutoAnnotatorInnerAmino">10 (2.0%)</td></tr><tr><td class="AutoAnnotatorInnerAmino">Val (V)</td><td class="AutoAnnotatorInnerAmino">33 (6.6%)</td></tr></table></td></tr></table></td></tr><tr><td class="AutoAnnotatorAminoCountingOuter"><strong>Amino acid counting</strong><table class="AutoAnnotatorNoBorder"><tr><td class="AutoAnnotatorAminoCountingInner1"></td><td class="AutoAnnotatorAminoCountingInner2">Total number:</td><td class="AutoAnnotatorAminoCountingInner3">498</td></tr><tr><td class="AutoAnnotatorAminoCountingInner1"></td><td class="AutoAnnotatorAminoCountingInner2">Positively charged (Arg+Lys):</td><td class="AutoAnnotatorAminoCountingInner3">36 (7.2%)</td></tr><tr><td class="AutoAnnotatorAminoCountingInner1"></td><td class="AutoAnnotatorAminoCountingInner2">Negatively charged (Asp+Glu):</td><td class="AutoAnnotatorAminoCountingInner3">53 (10.6%)</td></tr><tr><td class="AutoAnnotatorAminoCountingInner1"></td><td class="AutoAnnotatorAminoCountingInner2">Aromatic (Phe+His+Try+Tyr):</td><td class="AutoAnnotatorAminoCountingInner3">15 (3.0%)</td></tr></table></td><td class="AutoAnnotatorBiochemParOuter"><strong>Biochemical parameters</strong><table class="AutoAnnotatorNoBorder"><tr><td class="AutoAnnotatorBiochemParInner1"></td><td class="AutoAnnotatorBiochemParInner2">Atomic composition:</td><td class="AutoAnnotatorBiochemParInner3">C<sub>2163</sub>H<sub>3553</sub>N<sub>631</sub>O<sub>798</sub>S<sub>4</sub></td></tr><tr><td class="AutoAnnotatorBiochemParInner1"></td><td class="AutoAnnotatorBiochemParInner2">Molecular mass [Da]:</td><td class="AutoAnnotatorBiochemParInner3">51295.0</td></tr><tr><td class="AutoAnnotatorBiochemParInner1"></td><td class="AutoAnnotatorBiochemParInner2">Theoretical pI:</td><td class="AutoAnnotatorBiochemParInner3">4.50</td></tr><tr><td class="AutoAnnotatorBiochemParInner1"></td><td class="AutoAnnotatorBiochemParInner2">Extinction coefficient at 280 nm [M<sup>-1</sup> cm<sup>-1</sup>]:</td><td class="AutoAnnotatorBiochemParInner3">14900 / 14900 (all Cys red/ox)</td></tr></table></td></tr><tr><td class="AutoAnnotator1col" colspan="2"><strong>Plot for hydrophobicity, charge, predicted secondary structure, solvent accessability, transmembrane helices and disulfid bridges</strong> <input type='button' id='hydrophobicity_charge_button' onclick='show_or_hide_plot_1413480945739()' value='Show'><span id="hydrophobicity_charge_explanation"></span><div id="hydrophobicity_charge_container" style='display:none'><div id="hydrophobicity_charge_placeholder0" style="width:100%;height:150px"></div><div id="hydrophobicity_charge_placeholder1" style="width:100%;height:150px"></div><div id="hydrophobicity_charge_placeholder2" style="width:100%;height:150px"></div></div></td></tr><tr><td class="AutoAnnotator1col" colspan="2"><strong>Codon usage</strong><table class="AutoAnnotatorNoBorder"><tr><td class="AutoAnnotatorCodonUsage1"></td><td class="AutoAnnotatorCodonUsage2">Organism:</td><td class="AutoAnnotatorCodonUsage3"><i>E. coli</i></td><td class="AutoAnnotatorCodonUsage3"><i>B. subtilis</i></td><td class="AutoAnnotatorCodonUsage3"><i>S. cerevisiae</i></td><td class="AutoAnnotatorCodonUsage3"><i>A. thaliana</i></td><td class="AutoAnnotatorCodonUsage3"><i>P. patens</i></td><td class="AutoAnnotatorCodonUsage3">Mammals</td></tr><tr><td class="AutoAnnotatorCodonUsage1"></td><td class="AutoAnnotatorCodonUsage2">Codon quality (<a href="http://en.wikipedia.org/wiki/Codon_Adaptation_Index">CAI</a>):</td><td class="AutoAnnotatorCodonUsage3">excellent (0.83)</td><td class="AutoAnnotatorCodonUsage3">good (0.78)</td><td class="AutoAnnotatorCodonUsage3">good (0.68)</td><td class="AutoAnnotatorCodonUsage3">good (0.74)</td><td class="AutoAnnotatorCodonUsage3">excellent (0.83)</td><td class="AutoAnnotatorCodonUsage3">good (0.70)</td></tr></table></td></tr><tr><td class="AutoAnnotator1col" colspan="2"><strong>Alignments</strong> (obtained from <a href='http://predictprotein.org'>PredictProtein.org</a>)<table class="AutoAnnotatorNoBorder"><tr><td class="AutoAnnotatorAlignment1"></td><td class="AutoAnnotatorAlignment2">SwissProt:</td><td class="AutoAnnotatorAlignment3"><a href='http://www.uniprot.org/uniprot/P04949'>P04949</a> (100% identity on 498 AAs), <a href='http://www.uniprot.org/uniprot/P06179'>P06179</a> (47% identity on 495 AAs)</td></tr><tr><td class="AutoAnnotatorAlignment1"></td><td class="AutoAnnotatorAlignment2">TrEML:</td><td class="AutoAnnotatorAlignment3"><a href='http://www.uniprot.org/uniprot/L2Z8U7'>L2Z8U7</a> (100% identity on 498 AAs), <a href='http://www.uniprot.org/uniprot/M2PKZ4'>M2PKZ4</a> (100% identity on 498 AAs), <a href='http://www.uniprot.org/uniprot/C6EBD8'>C6EBD8</a> (100% identity on 498 AAs), <a href='http://www.uniprot.org/uniprot/C9QSQ0'>C9QSQ0</a> (100% identity on 498 AAs), <a href='http://www.uniprot.org/uniprot/F9QUT2'>F9QUT2</a> (100% identity on 498 AAs), <a href='http://www.uniprot.org/uniprot/G2EY21'>G2EY21</a> (100% identity on 498 AAs), <a href='http://www.uniprot.org/uniprot/I0ZSG6'>I0ZSG6</a> (100% identity on 498 AAs), <a href='http://www.uniprot.org/uniprot/C4ZQK1'>C4ZQK1</a> (100% identity on 498 AAs), <a href='http://www.uniprot.org/uniprot/Q53ZW4'>Q53ZW4</a> (100% identity on 498 AAs), <a href='http://www.uniprot.org/uniprot/U6N709'>U6N709</a> (100% identity on 498 AAs)</td></tr><tr><td class="AutoAnnotatorAlignment1"></td><td class="AutoAnnotatorAlignment2">PDB:</td><td class="AutoAnnotatorAlignment3"> - </td></tr></table></td></tr><tr><th id='AutoAnnotatorHeader' colspan="2"><strong>Predictions</strong> (obtained from <a href='http://predictprotein.org'>PredictProtein.org</a>)</th></tr><tr><td class="AutoAnnotatorLocalizationOuter"><strong>Subcellular Localization</strong> (reliability in brackets)<table class="AutoAnnotatorNoBorder"><tr><td class="AutoAnnotatorLocalization1"></td><td class="AutoAnnotatorLocalization2">Archaea:</td><td class="AutoAnnotatorLocalization3">cytosol (100%)</td></tr><tr><td class="AutoAnnotatorLocalization1"></td><td class="AutoAnnotatorLocalization2">Bacteria:</td><td class="AutoAnnotatorLocalization3">secreted (65%)</td></tr><tr><td class="AutoAnnotatorLocalization1"></td><td class="AutoAnnotatorLocalization2">Eukarya:</td><td class="AutoAnnotatorLocalization3">nucleus (55%)</td></tr></table></td><td class="AutoAnnotatorGOOuter"><strong>Gene Ontology</strong> (reliability in brackets)<br><table class="AutoAnnotatorNoBorder"><tr><td class='AutoAnnotatorGO1'></td><td class='AutoAnnotatorGO2'>Molecular Function Ontology:</td><td class='AutoAnnotatorGO3'><a href='http://amigo.geneontology.org/cgi-bin/amigo/term_details?term=GO:0005515'>GO:0005515</a> (100%)</td></tr><tr><td class='AutoAnnotatorGO1'></td><td class='AutoAnnotatorGO2'>Biological Process Ontology:</td><td class='AutoAnnotatorGO3'><a href='http://amigo.geneontology.org/cgi-bin/amigo/term_details?term=GO:0009405'>GO:0009405</a> (9%), <a href='http://amigo.geneontology.org/cgi-bin/amigo/term_details?term=GO:0001539'>GO:0001539</a> (63%)</td></tr><tr><td class='AutoAnnotatorGO1'> </td><td class='AutoAnnotatorGO2'> </td><td class='AutoAnnotatorGO3'> </td></tr></table></td></tr><tr><td class="AutoAnnotator1col" colspan="2"><strong>Predicted features:</strong><table class="AutoAnnotatorNoBorder"><tr><td class="AutoAnnotatorPredFeat1"></td><td class="AutoAnnotatorPredFeat2a">Disulfid bridges:</td><td class="AutoAnnotatorPredFeat3"> - </td></tr><tr><td class="AutoAnnotatorPredFeat1"></td><td class="AutoAnnotatorPredFeat2a">Transmembrane helices:</td><td class="AutoAnnotatorPredFeat3"> - </td></tr></table></td></tr><tr><td class="AutoAnnotator1col" colspan="2"> The BioBrick-AutoAnnotator was created by <a href="http://2013.igem.org/Team:TU-Munich">TU-Munich 2013</a> iGEM team. For more information please see the <a href="http://2013.igem.org/Team:TU-Munich/Results/Software">documentation</a>.<br>If you have any questions, comments or suggestions, please leave us a <a href="http://2013.igem.org/Team:TU-Munich/Results/AutoAnnotator">comment</a>.</td></tr></table></div><br><!-- IMPORTANT: DON'T REMOVE THIS LINE, OTHERWISE NOT SUPPORTED FOR IE BEFORE 9 --><!--[if lte IE 8]><script language="javascript" type="text/javascript" src="http://2013.igem.org/Team:TU-Munich/excanvas.js"></script><![endif]--><script type='text/javascript' src='http://code.jquery.com/jquery-1.10.0.min.js'></script><script type='text/javascript' src='http://2013.igem.org/Team:TU-Munich/Flot.js?action=raw&ctype=text/js'></script><script>var jqAutoAnnotator = jQuery.noConflict(true);function show_or_hide_plot_1413480945739(){hydrophobicity_datapoints = [[2.5,1.78],[3.5,0.70],[4.5,0.20],[5.5,0.20],[6.5,-0.80],[7.5,-0.94],[8.5,-0.40],[9.5,0.50],[10.5,2.10],[11.5,2.12],[12.5,0.66],[13.5,0.12],[14.5,-1.34],[15.5,-1.34],[16.5,-1.90],[17.5,-1.98],[18.5,-1.98],[19.5,-1.98],[20.5,-3.04],[21.5,-1.98],[22.5,-0.44],[23.5,0.10],[24.5,0.64],[25.5,0.64],[26.5,1.18],[27.5,-0.28],[28.5,-1.02],[29.5,-0.10],[30.5,-0.10],[31.5,-1.16],[32.5,-0.54],[33.5,1.12],[34.5,-0.54],[35.5,0.52],[36.5,-0.02],[37.5,-0.10],[38.5,-0.50],[39.5,-0.38],[40.5,-1.98],[41.5,-1.98],[42.5,-1.46],[43.5,-1.46],[44.5,-0.76],[45.5,-0.76],[46.5,0.30],[47.5,0.84],[48.5,0.84],[49.5,0.22],[50.5,0.02],[51.5,0.22],[52.5,-0.82],[53.5,-1.34],[54.5,-1.34],[55.5,0.46],[56.5,-0.88],[57.5,-0.82],[58.5,0.10],[59.5,0.66],[60.5,-0.94],[61.5,0.20],[62.5,0.64],[63.5,-1.02],[64.5,-1.58],[65.5,-0.52],[66.5,-1.58],[67.5,-2.64],[68.5,-1.82],[69.5,-0.22],[70.5,-0.74],[71.5,0.80],[72.5,1.86],[73.5,1.24],[74.5,0.20],[75.5,0.22],[76.5,-1.32],[77.5,-1.76],[78.5,-0.70],[79.5,0.20],[80.5,0.18],[81.5,0.18],[82.5,1.16],[83.5,0.10],[84.5,-1.36],[85.5,-1.90],[86.5,-0.44],[87.5,-2.04],[88.5,-2.24],[89.5,-0.70],[90.5,-0.90],[91.5,-2.36],[92.5,-0.90],[93.5,-0.14],[94.5,-0.14],[95.5,0.06],[96.5,1.12],[97.5,0.22],[98.5,0.22],[99.5,-0.70],[100.5,-0.14],[101.5,-1.20],[102.5,-1.22],[103.5,-1.78],[104.5,-1.86],[105.5,-2.42],[106.5,-0.96],[107.5,-0.96],[108.5,-0.42],[109.5,0.64],[110.5,0.64],[111.5,-0.82],[112.5,-1.36],[113.5,-0.30],[114.5,-1.98],[115.5,-1.44],[116.5,-1.64],[117.5,-0.18],[118.5,-1.78],[119.5,-1.70],[120.5,-0.64],[121.5,-0.44],[122.5,-2.10],[123.5,-0.56],[124.5,-0.02],[125.5,-1.00],[126.5,-1.00],[127.5,-0.24],[128.5,-1.78],[129.5,-1.06],[130.5,-1.68],[131.5,-1.06],[132.5,-0.08],[133.5,-0.08],[134.5,0.20],[135.5,1.66],[136.5,2.10],[137.5,0.48],[138.5,0.48],[139.5,-0.44],[140.5,-1.36],[141.5,-1.34],[142.5,-1.34],[143.5,0.26],[144.5,-0.36],[145.5,0.64],[146.5,0.18],[147.5,1.32],[148.5,-0.28],[149.5,-0.28],[150.5,-1.82],[151.5,-2.44],[152.5,-2.94],[153.5,-1.34],[154.5,-0.78],[155.5,0.82],[156.5,0.82],[157.5,1.72],[158.5,0.04],[159.5,-0.52],[160.5,-0.52],[161.5,-0.52],[162.5,-0.92],[163.5,-0.92],[164.5,-0.36],[165.5,-0.50],[166.5,0.12],[167.5,0.52],[168.5,0.60],[169.5,0.66],[170.5,0.46],[171.5,0.38],[172.5,0.46],[173.5,0.38],[174.5,-0.24],[175.5,-1.50],[176.5,-2.04],[177.5,-3.02],[178.5,-1.40],[179.5,-0.84],[180.5,-0.28],[181.5,0.26],[182.5,0.76],[183.5,-0.40],[184.5,0.58],[185.5,0.58],[186.5,1.10],[187.5,1.30],[188.5,1.54],[189.5,1.06],[190.5,1.06],[191.5,0.56],[192.5,-0.14],[193.5,-0.76],[194.5,-1.82],[195.5,-0.78],[196.5,-1.42],[197.5,-0.52],[198.5,0.04],[199.5,0.66],[200.5,0.66],[201.5,1.30],[202.5,1.30],[203.5,1.28],[204.5,1.22],[205.5,-0.38],[206.5,0.12],[207.5,-0.28],[208.5,-0.26],[209.5,-0.82],[210.5,-0.26],[211.5,-0.70],[212.5,-1.14],[213.5,-1.14],[214.5,-1.14],[215.5,-1.32],[216.5,-0.88],[217.5,-0.96],[218.5,0.08],[219.5,0.08],[220.5,0.32],[221.5,0.80],[222.5,0.70],[223.5,-0.34],[224.5,-0.34],[225.5,-0.96],[226.5,-1.88],[227.5,-2.32],[228.5,-2.88],[229.5,-2.44],[230.5,-2.00],[231.5,-1.56],[232.5,-1.64],[233.5,-0.04],[234.5,0.08],[235.5,0.26],[236.5,-0.18],[237.5,-0.10],[238.5,-1.70],[239.5,-2.26],[240.5,-2.26],[241.5,-2.96],[242.5,-2.52],[243.5,-2.08],[244.5,-1.02],[245.5,-0.10],[246.5,0.54],[247.5,1.64],[248.5,2.26],[249.5,1.20],[250.5,-0.34],[251.5,-0.28],[252.5,-1.26],[253.5,-0.78],[254.5,-0.22],[255.5,0.86],[256.5,1.30],[257.5,1.30],[258.5,0.38],[259.5,0.88],[260.5,0.36],[261.5,0.36],[262.5,-0.20],[263.5,0.24],[264.5,-0.26],[265.5,0.72],[266.5,0.22],[267.5,0.22],[268.5,0.22],[269.5,-0.34],[270.5,-1.32],[271.5,-1.32],[272.5,-1.40],[273.5,-1.40],[274.5,-0.84],[275.5,-0.84],[276.5,0.20],[277.5,0.84],[278.5,0.32],[279.5,0.38],[280.5,0.44],[281.5,-0.60],[282.5,-0.78],[283.5,0.22],[284.5,-0.40],[285.5,0.58],[286.5,0.02],[287.5,-0.36],[288.5,-1.34],[289.5,-0.28],[290.5,-1.26],[291.5,-0.72],[292.5,0.34],[293.5,0.34],[294.5,0.34],[295.5,-0.28],[296.5,0.64],[297.5,0.20],[298.5,0.70],[299.5,1.18],[300.5,1.72],[301.5,1.72],[302.5,2.64],[303.5,1.50],[304.5,1.42],[305.5,0.88],[306.5,-0.58],[307.5,-1.58],[308.5,-1.58],[309.5,-2.42],[310.5,-2.42],[311.5,-2.42],[312.5,-2.40],[313.5,-2.32],[314.5,-2.38],[315.5,-1.94],[316.5,-0.88],[317.5,0.02],[318.5,-0.06],[319.5,-0.62],[320.5,-0.50],[321.5,-1.56],[322.5,-2.40],[323.5,-2.32],[324.5,-0.86],[325.5,-0.98],[326.5,0.08],[327.5,0.52],[328.5,0.52],[329.5,0.60],[330.5,0.16],[331.5,-0.90],[332.5,-1.40],[333.5,-0.84],[334.5,-1.76],[335.5,-0.70],[336.5,0.84],[337.5,0.82],[338.5,1.80],[339.5,1.10],[340.5,0.60],[341.5,0.66],[342.5,0.68],[343.5,-0.42],[344.5,0.22],[345.5,-0.34],[346.5,-1.40],[347.5,-1.42],[348.5,-1.24],[349.5,-0.74],[350.5,0.32],[351.5,0.32],[352.5,0.32],[353.5,0.08],[354.5,-0.42],[355.5,-0.42],[356.5,0.58],[357.5,-0.04],[358.5,1.04],[359.5,1.10],[360.5,0.66],[361.5,-0.88],[362.5,-0.80],[363.5,-1.64],[364.5,-2.34],[365.5,-2.40],[366.5,-2.40],[367.5,-0.86],[368.5,0.06],[369.5,0.14],[370.5,1.18],[371.5,1.18],[372.5,0.26],[373.5,-1.36],[374.5,-0.80],[375.5,-1.96],[376.5,-1.96],[377.5,-2.04],[378.5,-0.90],[379.5,-1.46],[380.5,-0.44],[381.5,-0.44],[382.5,-0.36],[383.5,-0.80],[384.5,-0.80],[385.5,-0.80],[386.5,-0.80],[387.5,-0.86],[388.5,-0.86],[389.5,0.60],[390.5,-0.30],[391.5,0.76],[392.5,0.82],[393.5,0.82],[394.5,-0.64],[395.5,-0.14],[396.5,0.26],[397.5,0.20],[398.5,0.64],[399.5,2.18],[400.5,2.18],[401.5,0.72],[402.5,0.78],[403.5,-0.36],[404.5,-1.34],[405.5,-1.84],[406.5,-1.84],[407.5,-2.08],[408.5,-0.54],[409.5,-1.18],[410.5,-0.68],[411.5,0.78],[412.5,0.40],[413.5,-1.06],[414.5,0.08],[415.5,0.62],[416.5,0.22],[417.5,0.76],[418.5,2.30],[419.5,1.24],[420.5,-0.44],[421.5,-0.24],[422.5,-0.98],[423.5,-1.98],[424.5,-1.44],[425.5,0.10],[426.5,-0.54],[427.5,0.72],[428.5,1.72],[429.5,1.18],[430.5,-0.28],[431.5,-1.10],[432.5,-0.70],[433.5,-2.24],[434.5,-1.70],[435.5,-0.64],[436.5,1.10],[437.5,0.20],[438.5,0.20],[439.5,1.12],[440.5,0.06],[441.5,-1.48],[442.5,-1.48],[443.5,-0.92],[444.5,-1.82],[445.5,-1.82],[446.5,-0.36],[447.5,-0.38],[448.5,-0.94],[449.5,-0.44],[450.5,-0.44],[451.5,-1.36],[452.5,-2.10],[453.5,-0.50],[454.5,-1.56],[455.5,-1.56],[456.5,-1.04],[457.5,-0.84],[458.5,-2.00],[459.5,-0.94],[460.5,-0.38],[461.5,-1.44],[462.5,0.10],[463.5,0.20],[464.5,-0.86],[465.5,-0.34],[466.5,0.20],[467.5,-1.42],[468.5,-0.90],[469.5,-0.90],[470.5,-0.38],[471.5,0.68],[472.5,0.76],[473.5,-0.30],[474.5,0.76],[475.5,-0.22],[476.5,-1.82],[477.5,-1.28],[478.5,0.26],[479.5,0.66],[480.5,1.10],[481.5,1.02],[482.5,1.54],[483.5,0.00],[484.5,-1.46],[485.5,-0.98],[486.5,-0.52],[487.5,-1.58],[488.5,-1.58],[489.5,-0.04],[490.5,-0.12],[491.5,0.04],[492.5,1.50],[493.5,2.96],[494.5,1.42],[495.5,0.58]];charge_datapoints = [[2.5,0.00],[3.5,0.00],[4.5,0.00],[5.5,0.00],[6.5,0.00],[7.5,0.00],[8.5,0.00],[9.5,0.00],[10.5,0.00],[11.5,0.00],[12.5,0.00],[13.5,0.00],[14.5,0.00],[15.5,0.00],[16.5,0.00],[17.5,0.20],[18.5,0.20],[19.5,0.20],[20.5,0.20],[21.5,0.20],[22.5,0.00],[23.5,0.00],[24.5,0.00],[25.5,0.00],[26.5,0.00],[27.5,-0.20],[28.5,0.00],[29.5,0.00],[30.5,0.00],[31.5,0.00],[32.5,0.20],[33.5,0.00],[34.5,0.20],[35.5,0.20],[36.5,0.20],[37.5,0.20],[38.5,0.20],[39.5,0.20],[40.5,0.00],[41.5,-0.20],[42.5,-0.20],[43.5,-0.20],[44.5,-0.40],[45.5,-0.20],[46.5,0.00],[47.5,0.00],[48.5,0.00],[49.5,0.00],[50.5,0.20],[51.5,0.20],[52.5,0.20],[53.5,0.20],[54.5,0.20],[55.5,0.00],[56.5,0.20],[57.5,0.20],[58.5,0.20],[59.5,0.20],[60.5,0.20],[61.5,0.00],[62.5,0.00],[63.5,0.20],[64.5,0.20],[65.5,0.20],[66.5,0.20],[67.5,0.00],[68.5,-0.20],[69.5,-0.20],[70.5,-0.20],[71.5,-0.20],[72.5,0.00],[73.5,0.00],[74.5,0.00],[75.5,0.00],[76.5,-0.20],[77.5,-0.20],[78.5,-0.20],[79.5,-0.20],[80.5,-0.20],[81.5,-0.20],[82.5,-0.20],[83.5,-0.20],[84.5,-0.20],[85.5,-0.20],[86.5,0.00],[87.5,0.00],[88.5,0.20],[89.5,0.20],[90.5,0.40],[91.5,0.20],[92.5,0.20],[93.5,0.00],[94.5,0.00],[95.5,-0.20],[96.5,0.00],[97.5,0.00],[98.5,0.00],[99.5,0.00],[100.5,0.00],[101.5,0.00],[102.5,0.00],[103.5,-0.20],[104.5,-0.20],[105.5,-0.40],[106.5,-0.40],[107.5,-0.40],[108.5,-0.20],[109.5,-0.20],[110.5,0.00],[111.5,-0.20],[112.5,-0.40],[113.5,-0.40],[114.5,-0.20],[115.5,-0.20],[116.5,0.20],[117.5,0.40],[118.5,0.20],[119.5,-0.20],[120.5,-0.20],[121.5,-0.60],[122.5,-0.40],[123.5,-0.20],[124.5,-0.00],[125.5,-0.00],[126.5,0.20],[127.5,-0.00],[128.5,-0.00],[129.5,-0.00],[130.5,-0.00],[131.5,-0.00],[132.5,-0.00],[133.5,-0.00],[134.5,-0.00],[135.5,-0.00],[136.5,-0.00],[137.5,0.20],[138.5,0.20],[139.5,0.20],[140.5,0.20],[141.5,0.20],[142.5,0.20],[143.5,0.20],[144.5,0.20],[145.5,0.20],[146.5,0.20],[147.5,-0.00],[148.5,-0.00],[149.5,-0.20],[150.5,-0.20],[151.5,-0.20],[152.5,-0.20],[153.5,-0.20],[154.5,-0.00],[155.5,-0.00],[156.5,-0.20],[157.5,-0.20],[158.5,-0.00],[159.5,-0.00],[160.5,-0.00],[161.5,-0.00],[162.5,-0.00],[163.5,-0.00],[164.5,-0.00],[165.5,-0.00],[166.5,0.20],[167.5,0.20],[168.5,-0.20],[169.5,-0.20],[170.5,-0.20],[171.5,-0.20],[172.5,-0.20],[173.5,0.20],[174.5,0.20],[175.5,0.20],[176.5,-0.00],[177.5,-0.00],[178.5,-0.20],[179.5,-0.20],[180.5,-0.20],[181.5,-0.00],[182.5,-0.00],[183.5,-0.00],[184.5,-0.00],[185.5,-0.00],[186.5,-0.00],[187.5,-0.00],[188.5,-0.00],[189.5,-0.00],[190.5,-0.00],[191.5,-0.00],[192.5,-0.00],[193.5,-0.00],[194.5,-0.00],[195.5,-0.00],[196.5,0.20],[197.5,0.20],[198.5,0.20],[199.5,0.20],[200.5,0.20],[201.5,-0.00],[202.5,-0.00],[203.5,-0.00],[204.5,-0.00],[205.5,-0.20],[206.5,-0.20],[207.5,-0.20],[208.5,-0.20],[209.5,-0.40],[210.5,-0.20],[211.5,-0.20],[212.5,-0.20],[213.5,-0.20],[214.5,-0.00],[215.5,-0.00],[216.5,-0.00],[217.5,-0.00],[218.5,-0.00],[219.5,-0.20],[220.5,-0.20],[221.5,-0.20],[222.5,-0.20],[223.5,-0.20],[224.5,-0.20],[225.5,-0.20],[226.5,-0.20],[227.5,-0.20],[228.5,-0.40],[229.5,-0.20],[230.5,-0.20],[231.5,-0.20],[232.5,-0.00],[233.5,0.20],[234.5,0.20],[235.5,0.20],[236.5,0.20],[237.5,-0.20],[238.5,-0.20],[239.5,-0.40],[240.5,-0.40],[241.5,-0.20],[242.5,-0.00],[243.5,-0.00],[244.5,0.20],[245.5,0.20],[246.5,-0.00],[247.5,-0.00],[248.5,-0.00],[249.5,-0.00],[250.5,-0.20],[251.5,-0.20],[252.5,-0.20],[253.5,-0.20],[254.5,-0.20],[255.5,-0.00],[256.5,-0.00],[257.5,-0.00],[258.5,-0.00],[259.5,-0.00],[260.5,-0.00],[261.5,-0.00],[262.5,-0.00],[263.5,-0.00],[264.5,-0.00],[265.5,-0.00],[266.5,-0.00],[267.5,-0.20],[268.5,-0.20],[269.5,-0.20],[270.5,-0.20],[271.5,-0.20],[272.5,0.20],[273.5,0.20],[274.5,0.20],[275.5,0.20],[276.5,0.20],[277.5,-0.00],[278.5,-0.00],[279.5,-0.00],[280.5,-0.00],[281.5,-0.00],[282.5,-0.00],[283.5,-0.00],[284.5,-0.00],[285.5,-0.00],[286.5,-0.20],[287.5,-0.20],[288.5,-0.20],[289.5,-0.20],[290.5,-0.20],[291.5,-0.00],[292.5,-0.00],[293.5,-0.00],[294.5,-0.00],[295.5,-0.00],[296.5,-0.00],[297.5,-0.00],[298.5,-0.00],[299.5,-0.00],[300.5,-0.00],[301.5,-0.00],[302.5,-0.00],[303.5,0.20],[304.5,0.20],[305.5,0.20],[306.5,-0.00],[307.5,-0.00],[308.5,-0.00],[309.5,-0.00],[310.5,-0.00],[311.5,-0.00],[312.5,-0.00],[313.5,-0.40],[314.5,-0.40],[315.5,-0.40],[316.5,-0.20],[317.5,-0.20],[318.5,0.20],[319.5,-0.00],[320.5,-0.00],[321.5,-0.00],[322.5,-0.00],[323.5,-0.20],[324.5,-0.00],[325.5,-0.00],[326.5,-0.00],[327.5,-0.00],[328.5,-0.20],[329.5,-0.20],[330.5,-0.20],[331.5,-0.40],[332.5,-0.40],[333.5,-0.20],[334.5,-0.20],[335.5,-0.20],[336.5,-0.00],[337.5,-0.00],[338.5,-0.00],[339.5,0.20],[340.5,0.20],[341.5,0.20],[342.5,0.20],[343.5,0.20],[344.5,-0.00],[345.5,-0.20],[346.5,-0.20],[347.5,-0.20],[348.5,-0.20],[349.5,-0.20],[350.5,-0.00],[351.5,-0.00],[352.5,-0.00],[353.5,-0.00],[354.5,-0.00],[355.5,-0.00],[356.5,-0.00],[357.5,0.20],[358.5,0.20],[359.5,0.20],[360.5,0.20],[361.5,-0.00],[362.5,-0.40],[363.5,-0.40],[364.5,-0.20],[365.5,-0.20],[366.5,-0.20],[367.5,-0.00],[368.5,-0.00],[369.5,-0.40],[370.5,-0.40],[371.5,-0.40],[372.5,-0.40],[373.5,-0.20],[374.5,-0.00],[375.5,-0.00],[376.5,-0.00],[377.5,-0.00],[378.5,-0.20],[379.5,-0.40],[380.5,-0.40],[381.5,-0.20],[382.5,-0.20],[383.5,-0.20],[384.5,-0.00],[385.5,-0.00],[386.5,-0.00],[387.5,-0.00],[388.5,-0.00],[389.5,-0.00],[390.5,-0.00],[391.5,-0.00],[392.5,-0.00],[393.5,-0.00],[394.5,-0.20],[395.5,-0.20],[396.5,-0.20],[397.5,-0.20],[398.5,-0.20],[399.5,-0.00],[400.5,-0.00],[401.5,-0.00],[402.5,-0.00],[403.5,0.20],[404.5,0.20],[405.5,0.20],[406.5,-0.00],[407.5,-0.00],[408.5,-0.20],[409.5,-0.00],[410.5,-0.00],[411.5,0.20],[412.5,-0.00],[413.5,-0.20],[414.5,-0.40],[415.5,-0.40],[416.5,-0.40],[417.5,-0.20],[418.5,-0.00],[419.5,-0.20],[420.5,-0.00],[421.5,-0.00],[422.5,0.20],[423.5,0.20],[424.5,0.40],[425.5,0.20],[426.5,0.20],[427.5,-0.00],[428.5,-0.00],[429.5,-0.00],[430.5,-0.00],[431.5,0.20],[432.5,0.20],[433.5,-0.00],[434.5,-0.00],[435.5,-0.00],[436.5,-0.20],[437.5,-0.20],[438.5,-0.00],[439.5,-0.00],[440.5,-0.00],[441.5,-0.00],[442.5,-0.00],[443.5,-0.00],[444.5,-0.00],[445.5,-0.00],[446.5,-0.00],[447.5,-0.00],[448.5,-0.20],[449.5,-0.20],[450.5,-0.20],[451.5,-0.20],[452.5,-0.00],[453.5,0.20],[454.5,0.20],[455.5,-0.00],[456.5,-0.00],[457.5,-0.40],[458.5,-0.40],[459.5,-0.40],[460.5,-0.20],[461.5,-0.40],[462.5,-0.20],[463.5,-0.20],[464.5,-0.20],[465.5,-0.20],[466.5,-0.00],[467.5,0.20],[468.5,0.20],[469.5,0.20],[470.5,0.20],[471.5,0.20],[472.5,-0.00],[473.5,-0.00],[474.5,-0.00],[475.5,-0.00],[476.5,-0.00],[477.5,-0.00],[478.5,-0.00],[479.5,-0.00],[480.5,-0.00],[481.5,0.20],[482.5,0.20],[483.5,0.20],[484.5,0.20],[485.5,0.20],[486.5,-0.00],[487.5,-0.00],[488.5,-0.00],[489.5,-0.00],[490.5,-0.00],[491.5,-0.00],[492.5,-0.00],[493.5,-0.00],[494.5,-0.00],[495.5,-0.00]];dis_datapoints = [];trans_datapoints = [];sec_helix_datapoints = [[9,32],[45,69],[72,99],[106,128],[405,428],[430,447],[450,453],[459,485],[490,496]];sec_strand_datapoints = [[2,6],[37,38],[135,139],[144,149],[155,163],[166,177],[184,200],[201,201],[203,221],[233,240],[243,252],[255,287],[294,299],[302,308],[318,323],[326,333],[339,342],[348,350],[358,373],[383,384],[391,400],[401,401]];acc_exposed_datapoints = [[1,1],[16,17],[19,20],[23,24],[26,27],[30,30],[56,56],[59,60],[66,66],[69,69],[87,87],[101,101],[107,107],[110,110],[114,114],[117,118],[121,121],[125,125],[128,128],[133,133],[143,143],[145,145],[151,151],[153,153],[161,162],[164,164],[166,166],[171,171],[174,174],[176,176],[178,178],[191,191],[193,193],[195,197],[199,199],[204,204],[206,206],[208,208],[210,217],[220,220],[228,229],[231,231],[235,235],[237,237],[240,242],[244,244],[259,259],[261,261],[263,263],[265,265],[267,267],[269,273],[275,275],[277,278],[280,281],[291,295],[298,298],[300,301],[303,304],[306,306],[308,309],[311,311],[313,314],[316,316],[321,324],[331,331],[333,333],[336,336],[342,343],[345,345],[348,349],[352,352],[354,354],[364,367],[369,369],[372,372],[374,374],[376,377],[379,380],[382,382],[387,387],[389,389],[391,391],[395,395],[397,397],[405,405],[409,409],[412,412],[416,416],[419,419],[423,423],[440,441],[443,444],[447,447],[450,450],[467,467],[487,487],[490,490],[494,494],[497,498]];acc_buried_datapoints = [[3,15],[18,18],[21,21],[25,25],[28,29],[32,35],[38,38],[41,41],[43,51],[54,54],[58,58],[61,61],[64,65],[67,68],[71,82],[85,85],[88,89],[92,93],[95,100],[102,103],[109,109],[112,113],[116,116],[119,120],[123,123],[126,127],[130,130],[132,132],[134,135],[137,140],[146,146],[148,150],[155,156],[158,158],[160,160],[165,165],[168,168],[170,170],[175,175],[181,182],[184,187],[198,198],[200,200],[203,203],[205,205],[209,209],[225,225],[232,233],[245,246],[248,248],[250,250],[255,255],[258,258],[274,274],[284,284],[286,286],[288,288],[327,328],[332,332],[337,337],[339,339],[341,341],[353,353],[356,356],[358,359],[361,361],[373,373],[383,383],[399,399],[401,403],[407,407],[410,411],[414,414],[417,418],[421,421],[424,426],[428,435],[437,439],[442,442],[445,446],[448,449],[452,452],[454,454],[456,456],[458,462],[465,466],[468,469],[471,471],[473,474],[476,483],[485,485],[489,489],[492,493],[495,496]];flot_plot_options = []; flot_plot_options[0] = {grid: {borderWidth: {top: 0,right: 0,bottom: 0,left: 0}},legend: {show: false},xaxes: [{show: true,min: 0,max: 200,ticks: [[0.5, '1'], [24.5, '25'], [49.5, '50'], [74.5, '75'], [99.5, '100'], [124.5, '125'], [149.5, '150'], [174.5, '175'], [199.5, '200']],tickLength: -5}],yaxes: [{show: true,ticks: [[0, '0'], [4.5,'hydro-<br>phobic '], [-4.5,'hydro-<br>philic ']],min: -4.5,max: +4.5,font: {size: 12,lineHeight: 14,style: 'italic',weight: 'bold',family: 'sans-serif',variant: 'small-caps',color: 'rgba(100,149,237,1)'}},{show: true,ticks: [[0, ''], [1,'positive<br> charge'], [-1,'negative<br> charge']],position: 'right',min: -1,max: 1,font: {size: 12,lineHeight: 14,style: 'italic',weight: 'bold',family: 'sans-serif',variant: 'small-caps',color: 'rgba(255,99,71,1)'}}]};number_of_plots = 3;for ( plot_num = 1 ; plot_num < number_of_plots ; plot_num ++){flot_plot_options[plot_num] = jqAutoAnnotator.extend(true, {} ,flot_plot_options[0]);flot_plot_options[plot_num].xaxes = [{min: plot_num*200,max: (plot_num + 1)*200,ticks: [ [plot_num*200 + 0.5, (plot_num*200 + 1).toString()], [plot_num*200 + 24.5, (plot_num*200 + 25).toString()], [plot_num*200 + 49.5, (plot_num*200 + 50).toString()], [plot_num*200 + 74.5, (plot_num*200 + 75).toString()], [plot_num*200 + 99.5, (plot_num*200 + 100).toString()], [plot_num*200 + 124.5, (plot_num*200 + 125).toString()], [plot_num*200 + 149.5, (plot_num*200 + 150).toString()], [plot_num*200 + 174.5, (plot_num*200 + 175).toString()], [plot_num*200 + 199.5, (plot_num*200 + 200).toString()] ],tickLength: -5}];};try {if( jqAutoAnnotator('#AutoAnnotator_container_1413480945739 #hydrophobicity_charge_button').val() =='Show' ){jqAutoAnnotator('#AutoAnnotator_container_1413480945739 #hydrophobicity_charge_container').css('display','block');jqAutoAnnotator('#AutoAnnotator_container_1413480945739 #hydrophobicity_charge_button').val('Hide');var description_html = '<div id=\'AutoAnnotator_plot_selectors\'>';description_html = description_html + '<br> <input type=\'checkbox\' id=\'hydrophobicity_checkbox\' checked=\'checked\'> Moving average over 5 amino acids for hydrophobicity (<img src=\'https://static.igem.org/mediawiki/2013/e/e9/TUM13_hydrophobicity_icon.png\' alt=\'blue graph\' height=\'10\'></img>)';description_html = description_html + '<br> <input type=\'checkbox\' id=\'charge_checkbox\' checked=\'checked\'> Moving average over 5 amino acids for charge (<img src=\'https://static.igem.org/mediawiki/2013/3/3e/TUM13_charge_icon.png\' alt=\'red graph\' height=\'10\'></img>)';description_html = description_html + '<br> <input type=\'checkbox\' id=\'dis_checkbox\' checked=\'checked\'> Predicted disulfid bridges (<img src=\'https://static.igem.org/mediawiki/2013/2/28/TUM13_dis_icon.png\' alt=\'yellow circle\' height=\'10\'></img>) with the number of the bridge in the center';description_html = description_html + '<br> <input type=\'checkbox\' id=\'trans_checkbox\' checked=\'checked\'> Predicted transmembrane helices (<img src=\'https://static.igem.org/mediawiki/2013/7/78/TUM13_trans_icon.png\' alt=\'turquois bars\' height=\'10\'></img>)';description_html = description_html + '<br> <input type=\'checkbox\' id=\'sec_checkbox\' checked=\'checked\'> Predicted secondary structure: Helices (<img src=\'https://static.igem.org/mediawiki/2013/b/bf/TUM13_helix_icon.png\' alt=\'violet bars\' height=\'10\'></img>) and beta-strands (<img src=\'https://static.igem.org/mediawiki/2013/b/bf/TUM13_strand_icon.png\' alt=\'yellow bars\' height=\'10\'></img>)';description_html = description_html + '<br> <input type=\'checkbox\' id=\'acc_checkbox\' checked=\'checked\'> Predicted solvent accessability: Exposed (<img src=\'https://static.igem.org/mediawiki/2013/1/16/TUM13_exposed_icon.png\' alt=\'blue bars\' height=\'10\'></img>) and buried (<img src=\'https://static.igem.org/mediawiki/2013/0/0b/TUM13_buried_icon.png\' alt=\'green bars\' height=\'10\'></img>) residues';description_html = description_html + '<br></div>';jqAutoAnnotator('#AutoAnnotator_container_1413480945739 #hydrophobicity_charge_explanation').html(description_html);plot_according_to_selectors_1413480945739();jqAutoAnnotator('#AutoAnnotator_container_1413480945739 #AutoAnnotator_plot_selectors').find('input').click(plot_according_to_selectors_1413480945739);}else{jqAutoAnnotator('#AutoAnnotator_container_1413480945739 #hydrophobicity_charge_container').css('display','none');jqAutoAnnotator('#AutoAnnotator_container_1413480945739 #hydrophobicity_charge_button').val('Show');jqAutoAnnotator('#AutoAnnotator_container_1413480945739 #hydrophobicity_charge_explanation').html('');}}catch(err){txt='There was an error with the button controlling the visibility of the plot.\n';txt=txt+'The originating error is:\n' + err + '\n\n';alert(txt);}};function plot_according_to_selectors_1413480945739(){try{var plot_datasets = [[],[]];if(jqAutoAnnotator('#AutoAnnotator_container_1413480945739 #hydrophobicity_checkbox').prop('checked') == true){plot_datasets[0] = { color: 'rgba(100,149,237,1)',data: hydrophobicity_datapoints,label: 'Hydrophobicity',lines: { show: true, fill: true, fillColor: 'rgba(100,149,237,0.1)' },yaxis: 1};}if(jqAutoAnnotator('#AutoAnnotator_container_1413480945739 #charge_checkbox').prop('checked') == true){plot_datasets[1] = {color: 'rgba(255,99,71,1)',data: charge_datapoints,label: 'Charge',lines: { show: true, fill: true, fillColor: 'rgba(255,99,71,0.1)' },yaxis: 2};}for (plot_num = 0 ; plot_num < number_of_plots ; plot_num ++){jqAutoAnnotator.plot('#AutoAnnotator_container_1413480945739 #hydrophobicity_charge_placeholder'+ plot_num.toString(), plot_datasets, flot_plot_options[plot_num] );}var screen_width = jqAutoAnnotator('canvas.flot-base').width(); var pos_of_first_tick = 46;var pos_of_last_tick = screen_width - 51;var tick_diff = (screen_width - 97)/199;if(jqAutoAnnotator('#AutoAnnotator_container_1413480945739 #dis_checkbox').prop('checked') == true){for ( j = 0 ; j < dis_datapoints.length ; j++ ){jqAutoAnnotator('#AutoAnnotator_container_1413480945739 #hydrophobicity_charge_placeholder' + Math.floor((dis_datapoints[j][0] - 1)/200) ).append('<div class=\'AutoAnnotator_dis\' style=\'left:' + ((pos_of_first_tick - 8 + (dis_datapoints[j][0] - 1)*tick_diff - Math.floor((dis_datapoints[j][0] - 1)/200)*200*tick_diff).toFixed(0)).toString() + 'px;\'><b>' + (j+1) + '</b></div>');jqAutoAnnotator('#AutoAnnotator_container_1413480945739 #hydrophobicity_charge_placeholder' + Math.floor((dis_datapoints[j][1] - 1)/200) ).append('<div class=\'AutoAnnotator_dis\' style=\'left:' + ((pos_of_first_tick - 8 + (dis_datapoints[j][1] - 1)*tick_diff - Math.floor((dis_datapoints[j][1] - 1)/200)*200*tick_diff).toFixed(0)).toString() + 'px;\'><b>' + (j+1) + '</b></div>');}}if(jqAutoAnnotator('#AutoAnnotator_container_1413480945739 #trans_checkbox').prop('checked') == true){for ( j = 0 ; j < trans_datapoints.length ; j++ ){jqAutoAnnotator('#AutoAnnotator_container_1413480945739 #hydrophobicity_charge_placeholder' + Math.floor((trans_datapoints[j][0] - 1)/200) ).append('<div class=\'AutoAnnotator_trans\' style=\'width:' + (((trans_datapoints[j][1] - trans_datapoints[j][0] + 1)*tick_diff).toFixed(0)).toString() + 'px ;left:' + ((pos_of_first_tick + (trans_datapoints[j][0] - 1.5)*tick_diff - Math.floor((trans_datapoints[j][0] - 1)/200)*200*tick_diff).toFixed(0)).toString() + 'px\'></div>');}}if(jqAutoAnnotator('#AutoAnnotator_container_1413480945739 #sec_checkbox').prop('checked') == true){for ( j = 0 ; j < sec_helix_datapoints.length ; j++ ){jqAutoAnnotator('#AutoAnnotator_container_1413480945739 #hydrophobicity_charge_placeholder' + Math.floor((sec_helix_datapoints[j][0] - 1)/200) ).append('<div class=\'AutoAnnotator_sec_helix\' style=\'width:' + (((sec_helix_datapoints[j][1] - sec_helix_datapoints[j][0] + 1)*tick_diff).toFixed(0)).toString() + 'px; left:' + ((pos_of_first_tick + (sec_helix_datapoints[j][0] - 1.5)*tick_diff - Math.floor((sec_helix_datapoints[j][0] - 1)/200)*200*tick_diff).toFixed(0)).toString() + 'px\'></div>');}for ( j = 0 ; j < sec_strand_datapoints.length ; j++ ){jqAutoAnnotator('#AutoAnnotator_container_1413480945739 #hydrophobicity_charge_placeholder' + Math.floor((sec_strand_datapoints[j][0] - 1)/200) ).append('<div class=\'AutoAnnotator_sec_strand\' style=\'width:' + (((sec_strand_datapoints[j][1] - sec_strand_datapoints[j][0] + 1)*tick_diff).toFixed(0)).toString() + 'px; left:' + ((pos_of_first_tick + (sec_strand_datapoints[j][0] - 1.5)*tick_diff - Math.floor((sec_strand_datapoints[j][0] - 1)/200)*200*tick_diff).toFixed(0)).toString() + 'px\'></div>');}}if(jqAutoAnnotator('#AutoAnnotator_container_1413480945739 #acc_checkbox').prop('checked') == true){for ( j = 0 ; j < acc_buried_datapoints.length ; j++ ){jqAutoAnnotator('#AutoAnnotator_container_1413480945739 #hydrophobicity_charge_placeholder' + Math.floor((acc_buried_datapoints[j][0] - 1)/200) ).append('<div class=\'AutoAnnotator_acc_buried\' style=\'width:' + (((acc_buried_datapoints[j][1] - acc_buried_datapoints[j][0] + 1)*tick_diff).toFixed(0)).toString() + 'px; left:' + ((pos_of_first_tick + (acc_buried_datapoints[j][0] - 1.5)*tick_diff - Math.floor((acc_buried_datapoints[j][0] - 1)/200)*200*tick_diff).toFixed(0)).toString() + 'px\'></div>');}for ( j = 0 ; j < acc_exposed_datapoints.length ; j++ ){jqAutoAnnotator('#AutoAnnotator_container_1413480945739 #hydrophobicity_charge_placeholder' + Math.floor((acc_exposed_datapoints[j][0] - 1)/200) ).append('<div class=\'AutoAnnotator_acc_exposed\' style=\'width:' + (((acc_exposed_datapoints[j][1] - acc_exposed_datapoints[j][0] + 1)*tick_diff).toFixed(0)).toString() + 'px; left:' + ((pos_of_first_tick + (acc_exposed_datapoints[j][0] - 1.5)*tick_diff - Math.floor((acc_exposed_datapoints[j][0] - 1)/200)*200*tick_diff).toFixed(0)).toString() + 'px\'></div>');}}}catch(err){txt='There was an error while drawing the selected elements for the plot.\n';txt=txt+'The originating error is:\n' + err + '\n\n';throw(txt);}}</script></html> | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 18:05, 16 October 2014
FliC
FliC encodes the major flagellar protein of E. coli and is thus fundamental to motility
For more information on the biobrick and methods used go to http://2014.igem.org/wiki/index.php?title=Team:Glasgow/Project/Mobility_Proteins#motA
| Protein data table for BioBrick BBa_K1463600 automatically created by the BioBrick-AutoAnnotator version 1.0 | ||||||||||||||||||||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Nucleotide sequence in RFC 10: (underlined part encodes the protein) ATGGCACAA ... CTCCAGGGTTAA ORF from nucleotide position 1 to 1494 (excluding stop-codon) | ||||||||||||||||||||||||||||||||||||||||||||||
Amino acid sequence: (RFC 25 scars in shown in bold, other sequence features underlined; both given below)
| ||||||||||||||||||||||||||||||||||||||||||||||
Sequence features: (with their position in the amino acid sequence, see the list of supported features)
| ||||||||||||||||||||||||||||||||||||||||||||||
Amino acid composition:
| ||||||||||||||||||||||||||||||||||||||||||||||
Amino acid counting
| Biochemical parameters
| |||||||||||||||||||||||||||||||||||||||||||||
| Plot for hydrophobicity, charge, predicted secondary structure, solvent accessability, transmembrane helices and disulfid bridges | ||||||||||||||||||||||||||||||||||||||||||||||
Codon usage
| ||||||||||||||||||||||||||||||||||||||||||||||
Alignments (obtained from PredictProtein.org)
| ||||||||||||||||||||||||||||||||||||||||||||||
| Predictions (obtained from PredictProtein.org) | ||||||||||||||||||||||||||||||||||||||||||||||
Subcellular Localization (reliability in brackets)
| Gene Ontology (reliability in brackets)
| |||||||||||||||||||||||||||||||||||||||||||||
Predicted features:
| ||||||||||||||||||||||||||||||||||||||||||||||
| The BioBrick-AutoAnnotator was created by TU-Munich 2013 iGEM team. For more information please see the documentation. If you have any questions, comments or suggestions, please leave us a comment. | ||||||||||||||||||||||||||||||||||||||||||||||
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1224
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 301
Illegal AgeI site found at 709 - 1000COMPATIBLE WITH RFC[1000]