Difference between revisions of "Part:BBa K1463600"
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<partinfo>BBa_K1463600 short</partinfo>
 
<partinfo>BBa_K1463600 short</partinfo>
  
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To make the fliC biobrick, fliC was amplified by PCR using the proofreading Phusion polymerase with DS941 genomic DNA as template. The forward primer incorporated the prefix and added the BBa_B0034 ribosome binding site (RBS) and a scar sequence just upstream of fliC. The reverse primer incorporated the suffix and removed one undesirable Pst1 restriction site in fliC.  
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FliC encodes the major flagellar protein of E. coli and is thus fundamental to motility
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https://static.igem.org/mediawiki/2014/thumb/d/d0/GU_Figure_1_swarm_M.png/310px-GU_Figure_1_swarm_M.png
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https://static.igem.org/mediawiki/2014/f/fc/GU_Figure_2_Motility_histogram.png
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
  

Revision as of 17:58, 16 October 2014

FliC

FliC encodes the major flagellar protein of E. coli and is thus fundamental to motility

310px-GU_Figure_1_swarm_M.png

GU_Figure_2_Motility_histogram.png



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1224
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 301
    Illegal AgeI site found at 709
  • 1000
    COMPATIBLE WITH RFC[1000]