Difference between revisions of "Part:BBa K1326002"

Revision as of 17:30, 16 October 2014

Plac + CTH1 + YCF54 + Plasto + ChlM

Composite part incorporating the genes comprising the middle third of the chlorophyll biosynthetic pathway. CTH1 (copper targeting protein) and YCF54 make up the oxidative cyclase pathway. Their proteins create the fifth ring in the chlorophyll molecule. Plastocyanin is a copper-containing chloroplast precursor protein. Copper is involved in electron transfer on plastocyanin's functional site between cytochrome f (from cyctochrome b6f complex, from photosystem II) and P700+ (from photosystem I). ChlM is shorthand for Magnesium-protoporphyrin O-methyltransferase, which catalyses the methylation of magnesium protoporphyrin IX., frming magnesium protoporphyrin IX monomethylester.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 513
Illegal BglII site found at 621
Illegal BglII site found at 2409
Illegal BamHI site found at 1476
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 2966
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 1638
Illegal BsaI.rc site found at 311
Illegal BsaI.rc site found at 467
Illegal BsaI.rc site found at 1088
Illegal BsaI.rc site found at 1947
Illegal BsaI.rc site found at 2070

Functional Parameters

Figure 1: Single and double restriction digests of Operon 2. Single digest shows entire plasmid length at 5.48 kB. Double digest shows bands at 3.28 and 2.20 kB, for the operon 2 fragment length and BioBrick plasmid backbone respectively.

@@ Line 3: / Line 3: @@
 Composite part incorporating the genes comprising the middle third of the chlorophyll biosynthetic pathway.
-CTH1 (copper targeting protein) and YCF54 make up the oxidative cyclase pathway. Their proteins create the fifth ring in the chlorophyll molecule.
+[https://parts.igem.org/wiki/index.php?title=Part:BBa_K1080005 CTH1] (copper targeting protein) and [https://parts.igem.org/wiki/index.php?title=Part:BBa_K1080010 YCF54] make up the oxidative cyclase pathway. Their proteins create the fifth ring in the chlorophyll molecule.
-Plastocyanin is a copper-containing chloroplast precursor protein. Copper is involved in electron transfer on plastocyanin's functional site between cytochrome f (from cyctochrome b6f complex, from photosystem II) and P700+ (from photosystem I).
+[https://parts.igem.org/wiki/index.php?title=Part:BBa_K1080006 Plastocyanin] is a copper-containing chloroplast precursor protein. Copper is involved in electron transfer on plastocyanin's functional site between cytochrome f (from cyctochrome b6f complex, from photosystem II) and P700+ (from photosystem I).
-ChlM is shorthand for Magnesium-protoporphyrin O-methyltransferase, which catalyses the methylation of magnesium protoporphyrin IX., frming magnesium protoporphyrin IX monomethylester.
+[https://parts.igem.org/wiki/index.php?title=Part:BBa_K1080004 ChlM] is shorthand for Magnesium-protoporphyrin O-methyltransferase, which catalyses the methylation of magnesium protoporphyrin IX., frming magnesium protoporphyrin IX monomethylester.
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