Difference between revisions of "Part:BBa J58105:Design"
Line 1: | Line 1: | ||
− | |||
__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_J58105 short</partinfo> | <partinfo>BBa_J58105 short</partinfo> | ||
Line 7: | Line 6: | ||
===Design Notes=== | ===Design Notes=== | ||
− | + | [[Image:vanbp2.png|thumb|300px|Open and closed conformation of synthetic vanilline binding protein designed using an automatic computational design procedure.]] | |
− | + | We have used a structure for the closed rbsB (D-ribose binding protein, periplasmic) which has an x-ray structure with pdb 2DRI (271 residues). | |
===Source=== | ===Source=== | ||
+ | We have followed the work of Prof. Hellinga [1] but using the methodology of [2]. | ||
− | + | ===References=== | |
+ | [1] L.L. Looger, M.A. Dwyer, J. Smith, H.W. Hellinga. Computational design of receptor and sensor proteins with novel functions. Nature, 423, 185-190 (2003). | ||
− | + | [2] A. Jaramillo, L. Wernisch, S. Hery and S.J. Wodak. Folding free energy function selects native-like protein sequences in the core but not on the surface. Proc. Natl. Acad. Sci. 99 (2002), 13554-13559. |
Revision as of 23:16, 29 October 2006
Synthetic periplasmic binding protein that docks a vanillin molecule
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We have used a structure for the closed rbsB (D-ribose binding protein, periplasmic) which has an x-ray structure with pdb 2DRI (271 residues).
Source
We have followed the work of Prof. Hellinga [1] but using the methodology of [2].
References
[1] L.L. Looger, M.A. Dwyer, J. Smith, H.W. Hellinga. Computational design of receptor and sensor proteins with novel functions. Nature, 423, 185-190 (2003).
[2] A. Jaramillo, L. Wernisch, S. Hery and S.J. Wodak. Folding free energy function selects native-like protein sequences in the core but not on the surface. Proc. Natl. Acad. Sci. 99 (2002), 13554-13559.