Difference between revisions of "Part:BBa I742130:Experience"
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | ||
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It gave a lower fluorescence than our designed reverse RBS [https://parts.igem.org/Part:BBa_K1463560 BBa_K1463560]. | It gave a lower fluorescence than our designed reverse RBS [https://parts.igem.org/Part:BBa_K1463560 BBa_K1463560]. | ||
| − | [[Image:reverse-rbs-rfp.jpg|thumb|500px|center|'''Fig. 1:''' Red fluorescence of colonies containing BBa_I742130 or K1463560 reversed RBSs downstream of rfp gene transcribed from the PhiC31 integrase switch [https://parts.igem.org/Part:BBa_K1463000 K1463000]]] | + | [[Image:reverse-rbs-rfp.jpg|thumb|500px|center|'''Fig. 1:''' Red fluorescence of colonies containing BBa_I742130 or K1463560 reversed RBSs "downstream" of the reversed rfp gene [https://parts.igem.org/Part:BBa_K1463520 BBa_K1463520] transcribed from the PhiC31 integrase switch [https://parts.igem.org/Part:BBa_K1463000 K1463000]]] |
Consistent with this result, BBa_I742130 had a lower predicted translation initiation rate than BBa_K1463560 as calculated by the Salis RBS calculator. | Consistent with this result, BBa_I742130 had a lower predicted translation initiation rate than BBa_K1463560 as calculated by the Salis RBS calculator. | ||
| − | BBa_I742130 translation initiation rate 495.33 | + | BBa_I742130 calculated translation initiation rate 495.33 |
| − | BBa_K1463560 translation initiation rate 12,204.43 | + | BBa_K1463560 calculated translation initiation rate 12,204.43 |
===User Reviews=== | ===User Reviews=== | ||
Revision as of 21:41, 16 October 2014
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_I742130
Glasgow 2014 experience
We tested this reversed RBS in TOP10 and DH5alpha cells upstream (OK downstream) of our reversed RFP (Red Fluorescent Protein) biobrick BBa_K1463520. This can be seen in Fig. 1 below.
It gave a lower fluorescence than our designed reverse RBS BBa_K1463560.
Consistent with this result, BBa_I742130 had a lower predicted translation initiation rate than BBa_K1463560 as calculated by the Salis RBS calculator.
BBa_I742130 calculated translation initiation rate 495.33
BBa_K1463560 calculated translation initiation rate 12,204.43
User Reviews
UNIQdc9e05fa894d0421-partinfo-00000000-QINU UNIQdc9e05fa894d0421-partinfo-00000001-QINU