Difference between revisions of "Part:BBa K1317002:Design"
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The gene was synthesized by ordering specific oligo with overlapping regions, and by adding a NheI site for the assembling. | The gene was synthesized by ordering specific oligo with overlapping regions, and by adding a NheI site for the assembling. | ||
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===Source=== | ===Source=== |
Revision as of 14:19, 16 October 2014
CDS of silk-like protein (SLP)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 312
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The gene was synthesized by ordering specific oligo with overlapping regions, and by adding a NheI site for the assembling.
Source
major ampullate spidroin 1 (MaSp1) gene from Nephila clavipes
References
[2] Shevchuk N.A. et al. Construction of long DNA molecules using long PCR-based fusion of several fragments simultaneously (2004) Nucleic Acids Res., 32(2), 19