Difference between revisions of "Part:BBa K1453000"
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<partinfo>BBa_K1453000 short</partinfo>
 
<partinfo>BBa_K1453000 short</partinfo>
  
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This part is designed as a scaffold of the first kind of connectee, which can anchor on the membrane.
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This part is designed as a scaffold of the first kind of '''connectee''', which can anchor on the membrane.
  
  
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The membrane anchor system (ssDsbA-Lgt) comes from iGEM12_SJTU-BioX-Shanghai BBa_K771000  
 
The membrane anchor system (ssDsbA-Lgt) comes from iGEM12_SJTU-BioX-Shanghai BBa_K771000  
 
In order to be used cooperatively with TAL-Protein DiRepeats (Bba_K747000 to Bba_K747095), the TAL USB also consists of T1 sequence, T14 sequence and two sites for type II restriction enzyme BsmBI.
 
In order to be used cooperatively with TAL-Protein DiRepeats (Bba_K747000 to Bba_K747095), the TAL USB also consists of T1 sequence, T14 sequence and two sites for type II restriction enzyme BsmBI.
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== '''Design''' ==
 
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When digested with BsmBI, this part can produce two sticky-ends. One can complement with the first TAL-Protein DiRepeat (Bba_K747000 to Bba_K747015) at 5` of the sequence, while the other can complement with the sixth TAL-Protein DiRepeat (Bba_K747080 to Bba_K747095) at 3`.
 
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With the rest of TAL-Protein DiRepeats (Bba_K747016 to Bba_K747079), users can synthesize a 14-nucleotide-long Transactivator-like (TAL) protein to recogize their own connector and design their own polymerization. (Golden Gate Cloning)
 
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More details about membrane anchor, please view http://2012.igem.org/Team:SJTU-BioX-Shanghai.
 
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More details about TAL and Golden Gate Cloning, please view http://2012.igem.org/Team:Freiburg.
 
  
  

Revision as of 12:53, 16 October 2014

ssDsbA-mRFP-Lgt-TAL USB-His Tag

This part is designed as a scaffold of the first kind of connectee, which can anchor on the membrane.


Structure

The membrane anchor system (ssDsbA-Lgt) comes from iGEM12_SJTU-BioX-Shanghai BBa_K771000 In order to be used cooperatively with TAL-Protein DiRepeats (Bba_K747000 to Bba_K747095), the TAL USB also consists of T1 sequence, T14 sequence and two sites for type II restriction enzyme BsmBI.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 612
    Illegal AgeI site found at 724
    Illegal AgeI site found at 1537
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1158