Difference between revisions of "Part:BBa K1433008"
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<ol><li> RBS: 6N, a suitable RBS in paper (Bonnet, et al. 2012).</li> | <ol><li> RBS: 6N, a suitable RBS in paper (Bonnet, et al. 2012).</li> | ||
<li>gp35: a serine integrase in Mycobacterium phage Bxb1.</li> | <li>gp35: a serine integrase in Mycobacterium phage Bxb1.</li> | ||
− | <li>terminator: BBa_B0015, a strong double terminator.</li> | + | <li>terminator: [https://parts.igem.org/Part:BBa_B0015 BBa_B0015], a strong double terminator.</li> |
</ol></p> | </ol></p> | ||
Revision as of 13:41, 16 October 2014
6N-gp35-Terminator
Bxb1 gp35 is a serine integrase in Mycobacterium phage Bxb1. This intergrase can exclusively catalyze site-specific recombination between attB and attP, the attachment sites on phage chromosome and host chromosome. This recombination results in the reverse of the sequence between attB and attP, changing the two sites to attL and attR at the meantime. This inversion can be reversible by appropriately controlling the conditional expression of integrase and an excisionase in Bxb1 named gp47 at certain ratio. We change start codon ATG to GTG for a appropriate expression quantity.
Composition
- RBS: 6N, a suitable RBS in paper (Bonnet, et al. 2012).
- gp35: a serine integrase in Mycobacterium phage Bxb1.
- terminator: BBa_B0015, a strong double terminator.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 20
Illegal NheI site found at 217 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 491
Illegal XhoI site found at 578 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1130
Illegal NgoMIV site found at 1217
Illegal AgeI site found at 267 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1325