Difference between revisions of "Part:BBa J58102"
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Many signalling cellular processes occur using a phosphorylation mechanism. In order to assemble a transmembranal pathway, that frees a phosphore, with a genetic network within the cell, we use the promoter pOmpR (R0082). In E. coli, the osmosensing machinery using the EnvZ membrane protein follows this mechanism. That promoter is regulated positively (activated) by the transcription factor OmpR-P. We put a well known protein downstream: GFP (E0040) with a RBS (B0034) and a Terminator (B0015). This composite part will be very useful to characterize many cellular biosensors. | Many signalling cellular processes occur using a phosphorylation mechanism. In order to assemble a transmembranal pathway, that frees a phosphore, with a genetic network within the cell, we use the promoter pOmpR (R0082). In E. coli, the osmosensing machinery using the EnvZ membrane protein follows this mechanism. That promoter is regulated positively (activated) by the transcription factor OmpR-P. We put a well known protein downstream: GFP (E0040) with a RBS (B0034) and a Terminator (B0015). This composite part will be very useful to characterize many cellular biosensors. | ||
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+ | '''By Valencia iGEM 2006''' | ||
+ | |||
+ | A. Aparici (2), MC. Aroca (2), J. Carrera (1), C. Edo (1,2), G. Fuertes (2), D. Jimenez (2), C. Mata (2), JV. Medrano (2), A. Montagud (2), C. Navarrete (2), E. Navarro (1), G. Rodrigo (1), M. Baguena (1), P. Tortosa (3), P. Fernandez-de-Cordoba (1), A. Ferrando (2), J. Salgado (2), J. Urchueguia (1), A. Jaramillo (3). | ||
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+ | 1 Universidad Politecnica de Valencia, Spain | ||
+ | |||
+ | 2 Universitat de Valencia, Spain | ||
+ | |||
+ | 3 Ecole Polytechnique, France | ||
+ | |||
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Revision as of 14:43, 26 October 2006
Promoter activated by OmpR-P with the reporter GFP
Many signalling cellular processes occur using a phosphorylation mechanism. In order to assemble a transmembranal pathway, that frees a phosphore, with a genetic network within the cell, we use the promoter pOmpR (R0082). In E. coli, the osmosensing machinery using the EnvZ membrane protein follows this mechanism. That promoter is regulated positively (activated) by the transcription factor OmpR-P. We put a well known protein downstream: GFP (E0040) with a RBS (B0034) and a Terminator (B0015). This composite part will be very useful to characterize many cellular biosensors.
By Valencia iGEM 2006
A. Aparici (2), MC. Aroca (2), J. Carrera (1), C. Edo (1,2), G. Fuertes (2), D. Jimenez (2), C. Mata (2), JV. Medrano (2), A. Montagud (2), C. Navarrete (2), E. Navarro (1), G. Rodrigo (1), M. Baguena (1), P. Tortosa (3), P. Fernandez-de-Cordoba (1), A. Ferrando (2), J. Salgado (2), J. Urchueguia (1), A. Jaramillo (3).
1 Universidad Politecnica de Valencia, Spain
2 Universitat de Valencia, Spain
3 Ecole Polytechnique, France
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 778