Difference between revisions of "Part:BBa J58100"
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Two heterologous prokariotic activators, the bacteriophage lambda cI protein, and the E. coli cyclic AMP receptor protein (CRP), were shown to activate transcription synergistically from an artificial promoter bearing binding site for both proteins. The synergy depends on a functional activation (positive control) surface on each activator. Thus, both proteins interact directly with RNA polymerase. | Two heterologous prokariotic activators, the bacteriophage lambda cI protein, and the E. coli cyclic AMP receptor protein (CRP), were shown to activate transcription synergistically from an artificial promoter bearing binding site for both proteins. The synergy depends on a functional activation (positive control) surface on each activator. Thus, both proteins interact directly with RNA polymerase. | ||
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| + | A. Aparici (2), MC. Aroca (2), J. Carrera (1), C. Edo (1,2), G. Fuertes (2), D. Jimenez (2), C. Mata (2), JV. Medrano (2), A. Montagud (2), C. Navarrete (2), E. Navarro (1), G. Rodrigo (1), M. Baguena (1), P. Tortosa (3), P. Fernandez-de-Cordoba (1), A. Ferrando (2), J. Salgado (2), J. Urchueguia (1), A. Jaramillo (3). | ||
| + | 1 Universidad Politecnica de Valencia, Spain | ||
| + | 2 Universitat de Valencia, Spain | ||
| + | 3 Ecole Polytechnique, France | ||
| + | |||
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Revision as of 14:37, 26 October 2006
AND-type promoter synergistically activated by cI and CRP
Two heterologous prokariotic activators, the bacteriophage lambda cI protein, and the E. coli cyclic AMP receptor protein (CRP), were shown to activate transcription synergistically from an artificial promoter bearing binding site for both proteins. The synergy depends on a functional activation (positive control) surface on each activator. Thus, both proteins interact directly with RNA polymerase.
A. Aparici (2), MC. Aroca (2), J. Carrera (1), C. Edo (1,2), G. Fuertes (2), D. Jimenez (2), C. Mata (2), JV. Medrano (2), A. Montagud (2), C. Navarrete (2), E. Navarro (1), G. Rodrigo (1), M. Baguena (1), P. Tortosa (3), P. Fernandez-de-Cordoba (1), A. Ferrando (2), J. Salgado (2), J. Urchueguia (1), A. Jaramillo (3). 1 Universidad Politecnica de Valencia, Spain 2 Universitat de Valencia, Spain 3 Ecole Polytechnique, France
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 11
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 11
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 11
- 1000COMPATIBLE WITH RFC[1000]