Difference between revisions of "Part:BBa K1373001:Design"

Revision as of 05:45, 16 October 2014

nadE with strong promter and strong RBS

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

cloning via omega-PCR

the promoter is from iGEM distribution kit and the nadE gene is from the genome of E.coli MG1655

@@ Line 7: / Line 7: @@
 ===Design Notes===
 cloning via omega-PCR
-overexpression intracellular redox state of EAC is one of the most important physiological traits of extracellular electron transfer efficiency.In particular, the NAD+(H) pool size plays a central role of most metabolic pathways. By overexpressing the NAD synthetase, encoded be gene nadE and catalyzes the final step in de novo synthesis and salvage pathway of NAD biosynthesis (Fig. 1), the NAD+ level is increased thereby up-regulating genes whose products catalyze NADH synthesis. Therefore the augmented pool size of NAD+(H) result in promotion of NADH(the carrier of electrons)level, leading to high generation of intracellular releasable electrons and better electricity performance of EAC
 ===Source===