Difference between revisions of "Part:BBa K1462230:Design"
 
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===Design Notes===
 
===Design Notes===
 
The n-butanol pathway in the cytoplasm is constructed as a control group to identify the effectiveness of the pathway directed to the mitochondria. Crt,originated from Clostridium beijerinckii,expresses crotonase, which converts HbCoA to CrCoA, the third step of the n-butanol pathway.This part is constructed in pUC57 individually at the beginning and it is assembled with Hbd(BBa_K1462220),Erg10 (BBa_K1462210) in plasmid 181 ,which is leucine auxotroph.
 
The n-butanol pathway in the cytoplasm is constructed as a control group to identify the effectiveness of the pathway directed to the mitochondria. Crt,originated from Clostridium beijerinckii,expresses crotonase, which converts HbCoA to CrCoA, the third step of the n-butanol pathway.This part is constructed in pUC57 individually at the beginning and it is assembled with Hbd(BBa_K1462220),Erg10 (BBa_K1462210) in plasmid 181 ,which is leucine auxotroph.
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[[File:pathway.png|100px|left|frame|Figure 1. The n-butanol biosynthetic pathway. Enzymes are from these organisms: Erg10, S. cerevisiae; Hbd, Crt, AdhE2, Clostridium beijerinckii; Ccr, Streptomyces collinus.]]
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===Source===
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Clostridium beijerinckii
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===References===
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Eric J Steen, et al. (2008). Metabolic engineering of Saccharomyces cerevisiae for theproduction of n-butanol, Microbial Cell Factories, 7:36.
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Latest revision as of 09:27, 17 October 2014

Gal1+Crt+cyc1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 150
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The n-butanol pathway in the cytoplasm is constructed as a control group to identify the effectiveness of the pathway directed to the mitochondria. Crt,originated from Clostridium beijerinckii,expresses crotonase, which converts HbCoA to CrCoA, the third step of the n-butanol pathway.This part is constructed in pUC57 individually at the beginning and it is assembled with Hbd(BBa_K1462220),Erg10 (BBa_K1462210) in plasmid 181 ,which is leucine auxotroph.

Figure 1. The n-butanol biosynthetic pathway. Enzymes are from these organisms: Erg10, S. cerevisiae; Hbd, Crt, AdhE2, Clostridium beijerinckii; Ccr, Streptomyces collinus.