Difference between revisions of "Part:BBa K1442308:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | Codon optimised | + | <p>Codon optimised using IDT codon optimiser</p> |
− | + | <p>This contains restriction sites in order to insert testing modules either side of the forwards GFP.</p> | |
− | + | <p>The stop codons were artificially added in order to definitively stop translation following this.</p> | |
===Source=== | ===Source=== |
Revision as of 22:48, 15 October 2014
GFP codon optimised for human cells
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 599
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 44
Design Notes
Codon optimised using IDT codon optimiser
This contains restriction sites in order to insert testing modules either side of the forwards GFP.
The stop codons were artificially added in order to definitively stop translation following this.
Source
Part BBa_E0040