Difference between revisions of "Part:BBa K1463560"

Revision as of 14:24, 16 October 2014

Reverse RBS

Reverse ribosome binding site designed using the Salis ribosome binding site calculator to have a strength of 12,204 when placed downstream of the reversed rfp part BBa_K1463520

Usage and Biology

This has been used to drive expression of the reversed RFP part BBa_K1463520 in our phiC31 integrase-based inversion switch BBa_K1463000.

It gave a higher level of fluorescence than a previous design for a reverse RBS BBa_I742130

It was designed using the Salis RBS calculator software using the RBS sequence NNNNNNNNNNCTCTAGTA (10 Ns followed by a reversed scar) upstream of the RFP open reading frame.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 5: / Line 5: @@
 <!-- Add more about the biology of this part here -->
 ===Usage and Biology===
 This has been used to drive expression of the reversed RFP part [https://parts.igem.org/Part:BBa_K1463520 BBa_K1463520] in our phiC31 integrase-based inversion switch [https://parts.igem.org/Part:BBa_K1463000 BBa_K1463000].
 It gave a higher level of fluorescence than a previous design for a reverse RBS [https://parts.igem.org/Part:BBa_I742130 BBa_I742130]
+It was designed using the Salis RBS calculator software using the RBS sequence NNNNNNNNNNCTCTAGTA (10 Ns followed by a reversed scar) upstream of the RFP open reading frame.
+[[File:Example.jpg]]
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