Difference between revisions of "Part:BBa K1469001:Experience"
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Part is user sequenced: no illegal restriction sites and no mutations | Part is user sequenced: no illegal restriction sites and no mutations | ||
| − | Protein expression was checked by SDS-PAGE and Western Blot.[http://2014.igem.org/Team:Saarland/4_step] | + | Protein expression was checked by SDS-PAGE and Western Blot.[http://2014.igem.org/Team:Saarland/4_step#WesternBlot] |
Localisation of a GFP tagged Has2 protein variant was checked by fluorescence microscopy.[http://2014.igem.org/Team:Saarland/4_step] | Localisation of a GFP tagged Has2 protein variant was checked by fluorescence microscopy.[http://2014.igem.org/Team:Saarland/4_step] | ||
Revision as of 13:32, 15 October 2014
Part is user sequenced: no illegal restriction sites and no mutations
Protein expression was checked by SDS-PAGE and Western Blot.[http://2014.igem.org/Team:Saarland/4_step#WesternBlot]
Localisation of a GFP tagged Has2 protein variant was checked by fluorescence microscopy.[http://2014.igem.org/Team:Saarland/4_step]
No catalytic activity probably due to misfolding and intracellular accumulation.[http://2014.igem.org/Team:Saarland/4_step]
Applications of BBa_K1469001
BioBrick is designed for production of high molecular mass hyaluronic acid (HMM-HA) in a Bacillus megaterium based expression system. HMM-HA is supposed to have inhibitory effects on cancer cell lines
User Reviews
UNIQ08e5e424461b43d9-partinfo-00000000-QINU UNIQ08e5e424461b43d9-partinfo-00000001-QINU