Difference between revisions of "Part:BBa K1469001:Experience"

(Applications of BBa_K1469001)
Line 2: Line 2:
 
      
 
      
 
Part is user sequenced: no illegal restriction sites, no mutations
 
Part is user sequenced: no illegal restriction sites, no mutations
 +
 
Protein expression was checked by SDS-PAGE and Western Blot
 
Protein expression was checked by SDS-PAGE and Western Blot
 +
 
Localisation of a GFP tagged Has2 protein variant was checked by fluorescence microscopy
 
Localisation of a GFP tagged Has2 protein variant was checked by fluorescence microscopy
 +
 
No catalytic activity probably due to misfolding and intracellular accumulation
 
No catalytic activity probably due to misfolding and intracellular accumulation
 
   
 
   

Revision as of 13:17, 15 October 2014


Part is user sequenced: no illegal restriction sites, no mutations

Protein expression was checked by SDS-PAGE and Western Blot

Localisation of a GFP tagged Has2 protein variant was checked by fluorescence microscopy

No catalytic activity probably due to misfolding and intracellular accumulation

Applications of BBa_K1469001

BioBrick is designed for production of high molecular mass hyaluronic acid (HMM-HA) in a Bacillus megaterium based expression system. HMM-HA is supposed to have inhibitory effects on cancer cell lines

User Reviews

UNIQ57e1e0eff0607617-partinfo-00000000-QINU UNIQ57e1e0eff0607617-partinfo-00000001-QINU