Difference between revisions of "Part:BBa K1480004"

Revision as of 05:00, 17 October 2014

TetR generator (Promoter J23106 )

This is a TetR protein generator regulated by the constitutive promoter J23106.

The protein has a LVA tag for degradation.

Usage and Biology

It can be used as a constitutive generator of TetR to prove the function of a gene (or sequence) regulated by tet promoter.

Results

It is shown the effect of 4 different TetR generators on a mCherry gene (K1140003) that is down regulated by ptet. To the right side are listed the names of the generators.

Here are the links for the other parts used.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1480004 short</partinfo>
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 The protein has a LVA tag for degradation.
+==Usage and Biology==
+It can be used as a constitutive generator of TetR to prove the function of a gene (or sequence) regulated by tet promoter.
+==Results==
+[[File:TetR repressors(FCB UANL 2014).JPG|thumb|center|400px|It is shown the effect of 4 different TetR generators on a mCherry gene (K1140003) that is down regulated by ptet. To the right side are listed the names of the generators.]]
+Here are the links for the other parts used.
+ [[Link:https://parts.igem.org/Part:BBa_K1140003|K1140003]]
+ [[Link:https://parts.igem.org/Part:BBa_K1480003|K1480003]]
+ [[Link:https://parts.igem.org/Part:BBa_K145201|k145201]]
+ [[Link:https://parts.igem.org/Part:BBa_K1480004|K1480004]]
+ [[Link:https://parts.igem.org/Part:BBa_K1480005|K1480005]]
 <!-- Add more about the biology of this part here