Difference between revisions of "Part:BBa K1475001:Design"
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[[File:2014SDUdis_to_non-dis_ratio.png]] | [[File:2014SDUdis_to_non-dis_ratio.png]] | ||
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| − | The figure shows the ratio of indispensable to dispensable amino acids to the human metabolism contained in OneProt in comparison to the ratio recommended by the | + | The figure shows the ratio of indispensable to dispensable amino acids to the human metabolism contained in OneProt in comparison to the ratio recommended by the World Health Organization (WHO). [1] |
[[File:2014SDUnon-dis_ratio.png]]<br> | [[File:2014SDUnon-dis_ratio.png]]<br> | ||
| − | The figure shows the ratio of indispensible amino acids in OneProt compared to the ratio recommended by the | + | The figure shows the ratio of indispensible amino acids in OneProt compared to the ratio recommended by the World Health Organization (WHO). [1] |
===Source=== | ===Source=== | ||
Latest revision as of 07:03, 15 October 2014
OneProt coding region
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The amino acid sequence has been designed by first combining the number of triplets needed to comply with the World Health Organization recommendations for indispensable amino acids within 0.2% [1]. Then the sequence was scrambled until no relation to any known toxic proteins or virulence factors could be found, this was done by searching the coding sequence using Phyre2 [6].
The figure shows the ratio of indispensable to dispensable amino acids to the human metabolism contained in OneProt in comparison to the ratio recommended by the World Health Organization (WHO). [1]
The figure shows the ratio of indispensible amino acids in OneProt compared to the ratio recommended by the World Health Organization (WHO). [1]
Source
The sequence is based on WHO guidelines for ratios of amino acids needed in a normal human diet. [1]
References
1. WHO/FAO/UNU Expert Consultation: Protein and Amino Acid Requirements in Human Nutrition. United Nations University, 2002. No. 935, p. 164. http://whqlibdoc.who.int/trs/who_trs_935_eng.pdf
6. (Kelley L.A. and Sternberg M.J.E., Protein structure prediction on the web: a case study using the Phyre server. Nature Protocols 4, 2009, p. 363 - 371. http://www.sbg.bio.ic.ac.uk/phyre2/html/nprot.2009.2.pdf)