Difference between revisions of "Part:BBa I7100:Experience"

Revision as of 16:41, 23 October 2006

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_I7100

User Reviews

UNIQ547d11a32aa147e1-partinfo-00000000-QINU

Antiquity

This review comes from the old result system and indicates that this part did not work in some test.

Kahaynes

This part is supposed to be in well 10K of iGEM 2006 DNA-2, but what is actually in this well is probably a completely different part. We cloned this part in E. coli strain JM109 and saw no GFP expression. The vector is supposed to be pSB3K3 (2750 bp) and the insert should be ~950 bp, but a restriction digest showed the vector to be > 3000 bp and the insert is > 1000 bp. This plasmid should convey Kan resistance only, but the clones are both Amp and Kan resistant.

The digests used to confirm the plasmids at each step looked clean (two clear bands) so the content of well 10K of iGEM 2006 DNA-2 is probably a BioBrick part, just not the right vector or part. The vector is probably pSB*AK*.

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UNIQ547d11a32aa147e1-partinfo-00000003-QINU

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 This part is supposed to be in well 10K of ''iGEM 2006 DNA-2'', but what is actually in this well is probably a completely different part. We cloned this part in ''E. coli'' strain JM109 and saw no GFP expression. The vector is supposed to be pSB3K3 (2750 bp) and the insert should be ~950 bp, but a restriction digest showed the vector to be > 3000 bp and the insert is > 1000 bp. This plasmid should convey Kan resistance only, but the clones are both Amp and Kan resistant.
+The digests used to confirm the plasmids at each step looked clean (two clear bands) so the content of well 10K of ''iGEM 2006 DNA-2'' is probably a BioBrick part, just not the right vector or part. The vector is probably pSB*AK*.
 |};
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