Difference between revisions of "Part:BBa K1350007"

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BBa_K805011 given by iGEM12_HUST-China had BamH I site. The site is cleared by introducing a silent mutation. GGATCC (874-879) has been changed to GGGTCC. A codon GGA(Gly) is changed to GGG(Gly).. Because of the degeneracy of codon,the different sequence can encode the same enzyme.
 
BBa_K805011 given by iGEM12_HUST-China had BamH I site. The site is cleared by introducing a silent mutation. GGATCC (874-879) has been changed to GGGTCC. A codon GGA(Gly) is changed to GGG(Gly).. Because of the degeneracy of codon,the different sequence can encode the same enzyme.
  
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K805011 SequenceAndFeatures</partinfo>
  
 
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Revision as of 17:22, 14 October 2014

Cel5A, endoglucanase(BBa_K805011 Improved)


The endoglucanase genes cel5A can hydrolyze β-1,4glucosan randomly and act on long cellulose chains. The main product is oligomeric glucose. It can digest glucosan to produce glucose monomer in high efficiency with synergy with β-1,4glucosidase.It shows high ability to hydrolyze the crystal cellulose but relative lower enzyme activity towards carboxymethyl cellulose, barley β-glucan, and PASC.

Usage and Biology

Different from BBa_K805011 given by iGEM12_HUST-China, we have done the mutation of an ? to ?? and change the sequence in a way. However,because of the degeneracy of codon,the different sequence can encode the same enzyme.

Improved endoglucanase Cel5A

BBa_K805011 given by iGEM12_HUST-China had BamH I site. The site is cleared by introducing a silent mutation. GGATCC (874-879) has been changed to GGGTCC. A codon GGA(Gly) is changed to GGG(Gly).. Because of the degeneracy of codon,the different sequence can encode the same enzyme.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 874
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 307
    Illegal NgoMIV site found at 763
    Illegal AgeI site found at 1027
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1024