Difference between revisions of "Part:BBa K1431834"

 
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<partinfo>BBa_K1431834 short</partinfo>
 
<partinfo>BBa_K1431834 short</partinfo>
  
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Team Uppsala 2012 chromoprotein attracts many interests because its more convenient than fluorescent protein to be use as a reporter. However, few characterized data can be found for these proteins. SUSTC-Shenzhen this year want to tackle with theses problems.
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We've constructed a series of plasmids which are all in the same pattern: a strong/weak promoter, a strong/weak RBS and a chromoprotein. We want to monitor the speed of expression of these plasmids in normal incubation conditions (like 37℃ overnight for LB agar plate and 37℃ 180rpm for LB broth). The expression speed and strength of the constructed biobricks will be carefully monitored and gave others a relative scale for using chromoprotein as a reporter gene.
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===Selective Figures===
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<center></center>
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<center>'''LB agar plate of Biobricks BBa_K1431812 transformed in BL21 after incubating 23h at 37℃'''</center>
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<center></center>
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<center>'''LB agar plate of Biobricks BBa_K1431812 transformed in DH5α after incubating 23h at 37℃'''</center>
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<center>center>
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<center>'''LB broth of Biobricks BBa_K1431812 after incubating 11h at 37℃,180rpm'''</center>
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 03:14, 18 October 2014

amajLime, yellow-green chromoprotein reporter system (Weak Promoter, Strong RBS)

Team Uppsala 2012 chromoprotein attracts many interests because its more convenient than fluorescent protein to be use as a reporter. However, few characterized data can be found for these proteins. SUSTC-Shenzhen this year want to tackle with theses problems.

We've constructed a series of plasmids which are all in the same pattern: a strong/weak promoter, a strong/weak RBS and a chromoprotein. We want to monitor the speed of expression of these plasmids in normal incubation conditions (like 37℃ overnight for LB agar plate and 37℃ 180rpm for LB broth). The expression speed and strength of the constructed biobricks will be carefully monitored and gave others a relative scale for using chromoprotein as a reporter gene.

Selective Figures

LB agar plate of Biobricks BBa_K1431812 transformed in BL21 after incubating 23h at 37℃


LB agar plate of Biobricks BBa_K1431812 transformed in DH5α after incubating 23h at 37℃


center> <center>LB broth of Biobricks BBa_K1431812 after incubating 11h at 37℃,180rpm

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]