Difference between revisions of "Part:BBa K1431824:Experience"

 
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
 
This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
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===Applications of BBa_K1431824===
 
===Applications of BBa_K1431824===
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After we constructed this part, we tranformed into BL21 & DH5α to test expression effeciency. In the meantime, we did a comparison between BBa_K1431824 and BBa_K1431834 whose RBS is BBa_B0034, a relatively strong RBS.
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==LB agar plate growth comparison==
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We spread the plate for BL21 at 10:00am on 15th, Oct and DH5α at 11:00am on 15th, Oct. The plate contains 34ug/ml chloramphenicol. Following pictures were taken every several hours after placing the plates into a 37℃ incubator.
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JA is J23100,JB is J23106.
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At 00:30 (16th,Oct.)
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<center>https://static.igem.org/mediawiki/parts/5/58/SUSTC_31_916-1.jpg</center>
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At 1:50  (16th,Oct.)
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<center>https://static.igem.org/mediawiki/parts/9/94/SUSTC_31_916-2.jpg</center>
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At 3:00  (16th,Oct.)
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<center>https://static.igem.org/mediawiki/parts/f/f6/SUSTC_31_916-3.jpg</center>
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At 6:00  (16th,Oct.)
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<center>https://static.igem.org/mediawiki/parts/5/56/SUSTC_31_916-4.jpg</center>
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At 7:40  (16th,Oct.)
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<center>https://static.igem.org/mediawiki/parts/c/c3/SUSTC_31_916-5.jpg</center>
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At 8:00  (16th,Oct.)
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<center>https://static.igem.org/mediawiki/parts/9/94/SUSTC_31_916-6.jpg</center>
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At 10:00 (16th,Oct.)
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<center>https://static.igem.org/mediawiki/parts/0/0b/SUSTC_31_916-7.jpg</center>
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At 11:20 (16th,Oct.)
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<center>https://static.igem.org/mediawiki/parts/f/f3/SUSTC_31_916-8.jpg</center>
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==LB broth growth comparison==
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We isolated single colonies from the plates and incubate in 2ml LB broth with 34ug/ml chloramphenicol.
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Then we isolated single colonies from the plates.
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At 00:50 (17th,Oct.)
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<center>https://static.igem.org/mediawiki/parts/f/fb/SUSTC_31_916_L-1.jpg</center>
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At 02:45 (17th,Oct.)
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<center>https://static.igem.org/mediawiki/parts/3/33/SUSTC_31_916_L-2.jpg</center>
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At 04:55 (17th,Oct.)
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<center>https://static.igem.org/mediawiki/parts/0/05/SUSTC_31_916_L-3.jpg </center>
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At 22:40 (17th,Oct.)
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<center>https://static.igem.org/mediawiki/parts/9/95/SUSTC_31_916_L-4.jpg </center>
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==Conclusion==
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'''With observation, expression of BBa_K1431834 is stronger than BBa_K1431824.
  
 
===User Reviews===
 
===User Reviews===

Revision as of 02:14, 18 October 2014

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1431824

After we constructed this part, we tranformed into BL21 & DH5α to test expression effeciency. In the meantime, we did a comparison between BBa_K1431824 and BBa_K1431834 whose RBS is BBa_B0034, a relatively strong RBS.

LB agar plate growth comparison

We spread the plate for BL21 at 10:00am on 15th, Oct and DH5α at 11:00am on 15th, Oct. The plate contains 34ug/ml chloramphenicol. Following pictures were taken every several hours after placing the plates into a 37℃ incubator.

JA is J23100,JB is J23106.

At 00:30 (16th,Oct.)

SUSTC_31_916-1.jpg


At 1:50 (16th,Oct.)

SUSTC_31_916-2.jpg


At 3:00 (16th,Oct.)

SUSTC_31_916-3.jpg


At 6:00 (16th,Oct.)

SUSTC_31_916-4.jpg


At 7:40 (16th,Oct.)

SUSTC_31_916-5.jpg


At 8:00 (16th,Oct.)

SUSTC_31_916-6.jpg


At 10:00 (16th,Oct.)

SUSTC_31_916-7.jpg


At 11:20 (16th,Oct.)

SUSTC_31_916-8.jpg

LB broth growth comparison

We isolated single colonies from the plates and incubate in 2ml LB broth with 34ug/ml chloramphenicol. Then we isolated single colonies from the plates.

At 00:50 (17th,Oct.)

SUSTC_31_916_L-1.jpg


At 02:45 (17th,Oct.)

SUSTC_31_916_L-2.jpg


At 04:55 (17th,Oct.)

SUSTC_31_916_L-3.jpg


At 22:40 (17th,Oct.)

SUSTC_31_916_L-4.jpg

Conclusion

With observation, expression of BBa_K1431834 is stronger than BBa_K1431824.

User Reviews

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