Difference between revisions of "Part:BBa K1412088"

Revision as of 08:58, 16 October 2014

A combination of theophylline aptamer and taRNA that can response theophylline to regulate circuit

this part was made from the riboregulator taRNA by Isaacs et al(1)and theophylline aptamer by Lynch et al(2).Based on our desigen, the sequence of taRNA change some bases, while theophylline aptamer in our project is the same as Justin P.Gallivan group(2). Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1412088 short</partinfo>
+this part was made from the riboregulator taRNA by Isaacs et al(1)and theophylline aptamer by Lynch et al(2).Based on our desigen, the sequence of taRNA '''change some bases''', while theophylline aptamer in our project is the same as Justin P.Gallivan group(2).
 <!-- Add more about the biology of this part here
 ===Usage and Biology===
+Fabrication notes:
+In order to response difference molecular to regulate the gene circuit, we add theophylline aptamer based on the sequence of taRNA. When this piece is transcribed into mRNA,the aptamer's second structure complement with taRNA in term of the optimal configuration.When theophylline add into medium, aptamer can specificity combine it,changing the second structure,leading to the taRNA exposure.Now the key play a role to "wake up" our project lock.
+Part is also available with a constitutively on promoter as BBa_R0040, which is promoter tetR.
 <!-- -->