Difference between revisions of "Part:pSB1A3:Experience"
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Our team ([http://parts.mit.edu/wiki/index.php/Davidson_2006 Davidson College]) has used this vector in ''E. coli'' strain JM109 extensively. Clones are Amp resistant and are recovered in reasonable yields from mini preps. One drawback is that the vector has read-through transcription that comes into the cloned BioBrick part from both directions. This problem is a major one when an "off state" is critical for the control of a device. Parts without a promoter are expressed in this vector, regardless of orientation (see [https://parts.igem.org/wiki/index.php/Part:BBa_J31009:Design pSB1A7 Part Design] for details). Reverse read-through might be from the Amp resistance gene. Forward read-through might be from a cryptic promoter, since no known genes in the backbone are in the plus orientation. | Our team ([http://parts.mit.edu/wiki/index.php/Davidson_2006 Davidson College]) has used this vector in ''E. coli'' strain JM109 extensively. Clones are Amp resistant and are recovered in reasonable yields from mini preps. One drawback is that the vector has read-through transcription that comes into the cloned BioBrick part from both directions. This problem is a major one when an "off state" is critical for the control of a device. Parts without a promoter are expressed in this vector, regardless of orientation (see [https://parts.igem.org/wiki/index.php/Part:BBa_J31009:Design pSB1A7 Part Design] for details). Reverse read-through might be from the Amp resistance gene. Forward read-through might be from a cryptic promoter, since no known genes in the backbone are in the plus orientation. | ||
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| + | --[[User:Kahaynes|Kahaynes]] 15:48, 23 October 2006 (EDT) | ||
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Revision as of 19:48, 23 October 2006
This experience page is provided so that any user may enter their experience using this part.
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how you used this part and how it worked out.
Applications of pSB1A3
User Reviews
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Antiquity |
This review comes from the old result system and indicates that this part worked in some test. |
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Kahaynes |
Our team ([http://parts.mit.edu/wiki/index.php/Davidson_2006 Davidson College]) has used this vector in E. coli strain JM109 extensively. Clones are Amp resistant and are recovered in reasonable yields from mini preps. One drawback is that the vector has read-through transcription that comes into the cloned BioBrick part from both directions. This problem is a major one when an "off state" is critical for the control of a device. Parts without a promoter are expressed in this vector, regardless of orientation (see pSB1A7 Part Design for details). Reverse read-through might be from the Amp resistance gene. Forward read-through might be from a cryptic promoter, since no known genes in the backbone are in the plus orientation. --Kahaynes 15:48, 23 October 2006 (EDT) |
UNIQdcc049c71cdda486-partinfo-00000003-QINU