Difference between revisions of "Part:BBa K1424000"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K1424000 short</partinfo> | <partinfo>BBa_K1424000 short</partinfo> | ||
+ | |||
+ | Synechocystis sp. PCC 6803 possesses a annotated open reading frame (slr0168) with no known function. This open reading frame is used as a ‘neutral site’, i. e. a part of the genome that can be manipulated without apparent effect on the phenotype. For example this ‘neutral site’ has been used to evaluate mRNA expression of promoter probes using the lux and GFP system (Kunert et al, 2000). | ||
This is a prefix region of DNA for homologous recombination into a neutral site within the genome of Synechocystis sp. PCC 6803. | This is a prefix region of DNA for homologous recombination into a neutral site within the genome of Synechocystis sp. PCC 6803. | ||
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[[File:Conformation.jpg]] | [[File:Conformation.jpg]] | ||
+ | ===Usage and Biology=== | ||
+ | ADVANTAGES IN GENOME ENGINEERING APPLICATIONS: This part can be used to integrate exogenous DNA into the genome of Synechocystis sp. PCC 6803 at the site of gene slr0168 (gene of unknown function). | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Revision as of 09:50, 14 October 2014
Prefix homologous recombination for Synechocystis ("Left flank")
Synechocystis sp. PCC 6803 possesses a annotated open reading frame (slr0168) with no known function. This open reading frame is used as a ‘neutral site’, i. e. a part of the genome that can be manipulated without apparent effect on the phenotype. For example this ‘neutral site’ has been used to evaluate mRNA expression of promoter probes using the lux and GFP system (Kunert et al, 2000).
This is a prefix region of DNA for homologous recombination into a neutral site within the genome of Synechocystis sp. PCC 6803.
to be used with BBa_K1424001
Usage and Biology
ADVANTAGES IN GENOME ENGINEERING APPLICATIONS: This part can be used to integrate exogenous DNA into the genome of Synechocystis sp. PCC 6803 at the site of gene slr0168 (gene of unknown function).
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 441
- 1000COMPATIBLE WITH RFC[1000]