Difference between revisions of "Part:BBa K1424001"
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<partinfo>BBa_K1424001 short</partinfo>
 
<partinfo>BBa_K1424001 short</partinfo>
  
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This is a suffix region of DNA for homologous recombination into a neutral site within the genome of Synechocystis sp. PCC 6803.
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Synechocystis sp. PCC 6803 possesses a annotated open reading frame (slr0168) with no known function. This open reading frame is used as a ‘neutral site’, i. e. a part of the genome that can be manipulated without apparent effect on the phenotype. For example this ‘neutral site’ has been used to evaluate mRNA expression of promoter probes using the lux and GFP system (Kunert et al, 2000).
  
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To be used with BBa_K1424000
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This is a prefix region of DNA for homologous recombination into a neutral site within the genome of Synechocystis sp. PCC 6803.
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 +
to be used with BBa_K1424000  
 +
 
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[[File:Conformation.jpg‎]]
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===Usage and Biology===
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ADVANTAGES IN GENOME ENGINEERING APPLICATIONS: This part can be used to integrate exogenous DNA into the genome of Synechocystis sp. PCC 6803 at the site of gene slr0168 (gene of unknown function).
  
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[[File:conformation.jpg]]
 
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 09:51, 14 October 2014

Suffix homologous recombination for Synechocystis ("Right flank")

Synechocystis sp. PCC 6803 possesses a annotated open reading frame (slr0168) with no known function. This open reading frame is used as a ‘neutral site’, i. e. a part of the genome that can be manipulated without apparent effect on the phenotype. For example this ‘neutral site’ has been used to evaluate mRNA expression of promoter probes using the lux and GFP system (Kunert et al, 2000).

This is a prefix region of DNA for homologous recombination into a neutral site within the genome of Synechocystis sp. PCC 6803.

to be used with BBa_K1424000

Conformation.jpg

Usage and Biology

ADVANTAGES IN GENOME ENGINEERING APPLICATIONS: This part can be used to integrate exogenous DNA into the genome of Synechocystis sp. PCC 6803 at the site of gene slr0168 (gene of unknown function).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]