Difference between revisions of "Part:BBa K1520018"

Revision as of 05:02, 14 October 2014

Prlux-rbs-cI-Ter-PcI-rbs-lacI-Ter

Plasmid 2 in detector: Prlux-rbs-cI-Ter-PcI-rbs-lacI-Ter It is a low level detection plasmid. It suppresses the expression of lacI when AHL is enough, which will result in the expression of rfp when AHL is above a certain level.

This plasmid can also help to suppress the expression of rfp in the plasmid 1, which will end up making the bacteria with two kinds of plasmids can express rfp at a certain AHL concentration.

File:Detectiontogether.jpg

The left one is the bacteria with plasmid 1, the middle one is the bacteria with plasmid 2, the right is the bacteria with plasmid 1&2

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 2223
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K1520018 short</partinfo>
-Plasmid2 in detector:
+Plasmid 2 in detector:
 Prlux-rbs-cI-Ter-PcI-rbs-lacI-Ter
 It is a low level detection plasmid. It suppresses the expression of lacI when AHL is enough, which will result in the expression of rfp when AHL is above a certain level.
 [[File:AHLbelowT.jpg]]
+This plasmid can also help to suppress the expression of rfp in the plasmid 1, which will end up making the bacteria with two kinds of plasmids can express rfp at a certain AHL concentration.
+[[File:detectiontogether.jpg]]
+The left one is the bacteria with plasmid 1, the middle one is the bacteria with plasmid 2, the right is the bacteria with plasmid 1&2
 <!-- Add more about the biology of this part here