Difference between revisions of "Part:BBa K1017726:Experience"

Latest revision as of 21:34, 13 October 2014

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1017726

User Reviews

UNIQd14e5b882a8e6105-partinfo-00000000-QINU UNIQd14e5b882a8e6105-partinfo-00000001-QINU

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2014_WashU_StLouis

We used this part in order to activate the CcaR/CcaS light sensor system. Not 100% sure if the sequence is as depicted as we were able to get different results for our system with and without the chromophore plasmid; but when we tried to digest the part with known restriction enzymes as a crude verification of the part sequence, it would not digest properly. We also tried to amplify the backbone for biobricking purposes and would get proper piece lengths but improper sequence binding when running our sequencing PCR with primers that should have bound to the PSB1C3 backbone. Visit our project here: http://2014.igem.org/Team:WashU_StLouis

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 <I>2014_WashU_StLouis</I>
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-We used this part in order to activate the CcaR/CcaS light sensor system. Not 100% sure if it works as intended, as we were able to get different results for our system with and without the chromophore plasmid; but when we tried to digest the part with known restriction enzymes as a crude verification of the part sequence, it would not digest properly.
+We used this part in order to activate the CcaR/CcaS light sensor system. Not 100% sure if the sequence is as depicted as we were able to get different results for our system with and without the chromophore plasmid; but when we tried to digest the part with known restriction enzymes as a crude verification of the part sequence, it would not digest properly. We also tried to amplify the backbone for biobricking purposes and would get proper piece lengths but improper sequence binding when running our sequencing PCR with primers that should have bound to the PSB1C3 backbone.
 Visit our project here: http://2014.igem.org/Team:WashU_StLouis
 |};