Difference between revisions of "Part:BBa K1350003"
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•Fig. 2 shows the most suitable III-3-A enzyme pH is around 9, as a typical alkaline cellulase.For study on the stability of III-3-A pH, respectively save the pure enzyme solution under different predetermined pH for 30min, then according to the conventional method determine the enzyme activity under pH9.The results showed that, the enzyme in the pH 6-11 range,the enzyme activity can be maintained above 80%.Visible on the alkaline cellulase in weak acidic and alkaline conditions with strong stability. | •Fig. 2 shows the most suitable III-3-A enzyme pH is around 9, as a typical alkaline cellulase.For study on the stability of III-3-A pH, respectively save the pure enzyme solution under different predetermined pH for 30min, then according to the conventional method determine the enzyme activity under pH9.The results showed that, the enzyme in the pH 6-11 range,the enzyme activity can be maintained above 80%.Visible on the alkaline cellulase in weak acidic and alkaline conditions with strong stability. | ||
| + | <br>===Research on temperature stability of III -3-A enzyme=== | ||
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Revision as of 15:09, 13 October 2014
Alkaline Cellulase
Alkaline cellulase gene from bacillus subtilis saline lake monoclonal, it can survive in high alkalinity and high heat environment, and keep good enzyme activity.Therefore, in the industrial production, such as in the production of paper making, washing and so on, the alkaline cellulose enzyme can have higher, more extensive application range, and can greatly reduce the energy consumption and production cost.
Fig. 1Effect of pH on the activity and stability
•Fig. 2 shows the most suitable III-3-A enzyme pH is around 9, as a typical alkaline cellulase.For study on the stability of III-3-A pH, respectively save the pure enzyme solution under different predetermined pH for 30min, then according to the conventional method determine the enzyme activity under pH9.The results showed that, the enzyme in the pH 6-11 range,the enzyme activity can be maintained above 80%.Visible on the alkaline cellulase in weak acidic and alkaline conditions with strong stability.
===Research on temperature stability of III -3-A enzyme===
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]