Difference between revisions of "Part:BBa K1439000:Experience"
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | ||
how you used this part and how it worked out. | how you used this part and how it worked out. | ||
Revision as of 12:27, 16 October 2014
Test1. Conjugation between E.coli HB101 and Top10
This experience page is provided so that any user may enter their experience using this part.
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how you used this part and how it worked out.
Applications of BBa_K1439000
We have test the oriTRP4 BBa_K1439000 in a conjugation system in which pSB1C3 with the help of plasmid RP4, and E. coli HB101 as donor and E.coil Top10 as recipient cells. We test the conjugative competence of oriTRP4 BBa_K1439000 by conjugate between HB101 and Top10. We use the RFP (BBa_J04450) marks the plasmids containing oriTRP4 BBa_K1439000. We get HB101 without streptomycin resistance form Zhigang Qiu. Top 10 has streptomycin resistance. After conjugation, we select recipient cells by streptomycin resistance LB medium and statistical number of red bacterial colony.
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UNIQe552390e85ba7945-partinfo-00000000-QINU UNIQe552390e85ba7945-partinfo-00000001-QINU Test
Conjugate between E.coil HB101 and E.coil Top10.