Difference between revisions of "Part:BBa K1463000"

Revision as of 12:53, 13 October 2014

Recombinase Switch With GFP and RFP

A recombinase switch with GFP one side of the switch and RFP on the other.

The image below shows a schematic of the switch. The

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 813
Illegal NheI site found at 836
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 793
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 34
Illegal AgeI site found at 146
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 711
Illegal BsaI.rc site found at 1668

@@ Line 4: / Line 4: @@
 A recombinase switch with GFP one side of the switch and RFP on the other.
-[[Image:GU_jake_gel_1.jpg|thumb|500px|right|'''Fig. 1:''' 1.pZJ7 on its own, 2.Switch #2 on its own, 3.Switch #2 + pZJ7 glucose, 4.Switch #2 + pZJ7 arabinose, 5.Switch #3 on its own, 6.Switch #3 + pZJ7 glucose, 7.Switch #3 + pZJ7 arabinose, 8.Switch #4 on its own, 9.Switch #4 + pZJ7 glucose, 10.Switch #4 + pZJ7 arabinose, 11.pBAD33 gvpAC (ignore)12.1kb+ marker.]]
+[['''Fig. 1:''' 1.pZJ7 on its own, 2.Switch #2 on its own, 3.Switch #2 + pZJ7 glucose, 4.Switch #2 + pZJ7 arabinose, 5.Switch #3 on its own, 6.Switch #3 + pZJ7 glucose, 7.Switch #3 + pZJ7 arabinose, 8.Switch #4 on its own, 9.Switch #4 + pZJ7 glucose, 10.Switch #4 + pZJ7 arabinose, 11.pBAD33 gvpAC (ignore)12.1kb+ marker.]]
 https://static.igem.org/mediawiki/2014/f/f3/GU_jake_gel_1.jpg
+The image below shows a schematic of the switch. The