Difference between revisions of "Part:BBa K1463000"
Line 5: Line 5:
  
 
https://static.igem.org/mediawiki/2014/f/f3/GU_jake_gel_1.jpg
 
https://static.igem.org/mediawiki/2014/f/f3/GU_jake_gel_1.jpg
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1.pZJ7 on its own
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2.Switch #2 on its own
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3.Switch #2 + pZJ7 glucose
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4.Switch #2 + pZJ7 arabinose
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5.Switch #3 on its own
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6.Switch #3 + pZJ7 glucose
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7.Switch #3 + pZJ7 arabinose
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8.Switch #4 on its own
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9.Switch #4 + pZJ7 glucose
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10.Switch #4 + pZJ7 arabinose
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11.pBAD33 gvpAC (ignore)
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12.1kb+ marker
  
  

Revision as of 12:47, 13 October 2014

Recombinase Switch With GFP and RFP

A recombinase switch with GFP one side of the switch and RFP on the other.

GU_jake_gel_1.jpg 1.pZJ7 on its own 2.Switch #2 on its own 3.Switch #2 + pZJ7 glucose 4.Switch #2 + pZJ7 arabinose 5.Switch #3 on its own 6.Switch #3 + pZJ7 glucose 7.Switch #3 + pZJ7 arabinose 8.Switch #4 on its own 9.Switch #4 + pZJ7 glucose 10.Switch #4 + pZJ7 arabinose 11.pBAD33 gvpAC (ignore) 12.1kb+ marker


Switch_diagram_sites_small2.jpg

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 813
    Illegal NheI site found at 836
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 793
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 34
    Illegal AgeI site found at 146
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 711
    Illegal BsaI.rc site found at 1668