Difference between revisions of "Part:BBa K1463000"
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A recombinase switch with GFP one side of the switch and RFP on the other.
 
A recombinase switch with GFP one side of the switch and RFP on the other.
  
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[[Image:GU_jake_gel_1.jpg‎|thumb|500px|right|'''Fig. 1:''' BL21 ''E. coli'' carrying different constructs on [http://2012.igem.org/Team:Goettingen/Project/Materials#M9_Swimming_Agar M9 minimal medium swarming agar]. after 48h incubation at 33°C. Cells expressing ''fliC'' in puc18 under the natural promoter travelled approximately 1,5cm (radius) whereas no swimming could be detected for the control plasmid carrying [https://parts.igem.org/Part:BBa_K777125 K777125]. On tryptone swimming agar ''yhjH'' transformants were usually faster.]]
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https://static.igem.org/mediawiki/2014/f/f3/GU_jake_gel_1.jpg
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[[Image:GU_jake_gel_1.jpg‎|thumb|center|500px|Calibration curve of concentration of GFP against fluorescence. The conversion factor is 79.429.]]
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 12:27, 13 October 2014

Recombinase Switch With GFP and RFP

A recombinase switch with GFP one side of the switch and RFP on the other.

GU_jake_gel_1.jpg

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 813
    Illegal NheI site found at 836
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 793
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 34
    Illegal AgeI site found at 146
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 711
    Illegal BsaI.rc site found at 1668