Difference between revisions of "Part:BBa K1366102"
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This construct contains the sequence homologies to delete the msbB gene (Braun’s lipoprotein) with a kanamycin resistance and the sequences for the Lux I production (AHL synthase). The deletion of msbB gene and the replacement of that sequence with the Kan resistance and LuxI will be performed with the red-lambda system. The FTR sequences included are used to remove the antibiotic resistance with a specific recombinase.  
 
This construct contains the sequence homologies to delete the msbB gene (Braun’s lipoprotein) with a kanamycin resistance and the sequences for the Lux I production (AHL synthase). The deletion of msbB gene and the replacement of that sequence with the Kan resistance and LuxI will be performed with the red-lambda system. The FTR sequences included are used to remove the antibiotic resistance with a specific recombinase.  
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https://static.igem.org/mediawiki/2014/9/95/BB1_BB2_PNG.png
  
  

Revision as of 02:24, 18 October 2014

Genetic construct for msbB gene deletion (C2)

This construct contains the sequence homologies to delete the msbB gene (Braun’s lipoprotein) with a kanamycin resistance and the sequences for the Lux I production (AHL synthase). The deletion of msbB gene and the replacement of that sequence with the Kan resistance and LuxI will be performed with the red-lambda system. The FTR sequences included are used to remove the antibiotic resistance with a specific recombinase.

BB1_BB2_PNG.png


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1108
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 772
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 621
    Illegal SapI.rc site found at 831