Difference between revisions of "Part:BBa K1381017"

Revision as of 14:30, 12 October 2014

CFY-X6 his

Colicin Fy The CFY is a bacteriocin in the colicin family. It is produced naturally by Y.frederiksenii and target closed relatives to that bacteria. One among them is the Y.enterocolitica and our target pathogene. The Colicin Fy will target the cellmembrane of it's target.

The killing system We designed the killing system to secret the colicin Fy with the help of an export tag, the USP45. This part will be without promoter, RBS and export tag. It only contains the colicin Fy and a 6x His-Tag for characterization. It is designed so that you can easily couple another promoter or export tag of your choice. This part contains the restriction sites NgoMIV att the start of the sequence to more easily fuse proteins like an export tag to it. Since it got a NgoMIV site att the start of the sequence it is possible to fuse other sequence if they follow the Freiburg Biobrick standard 25 and contains an AgeI site. However our part is not entirely built in the Freiburg standard Biobrick 25 since it does not contain an AgeI site.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 4
1000
COMPATIBLE WITH RFC[1000]

Revision as of 14:20, 12 October 2014 (view source) Amaron (Talk \| contribs) ← Older edit		Revision as of 14:30, 12 October 2014 (view source) Amaron (Talk \| contribs) Newer edit →
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	'''The killing system'''		'''The killing system'''
−	We designed the killing system to secret the colicin Fy with the help of an export tag, the USP45. This part will be without promoter, RBS and export tag. It only contains the colicin Fy and a 6x His-Tag for characterization. It is designed so that you can easily couple another promoter or export tag of your choice.	+	We designed the killing system to secret the colicin Fy with the help of an export tag, the USP45. This part will be without promoter, RBS and export tag. It only contains the colicin Fy and a 6x His-Tag for characterization. It is designed so that you can easily couple another promoter or export tag of your choice. This part contains the restriction sites NgoMIV att the start of the sequence to more easily fuse proteins like an export tag to it. Since it got a NgoMIV site att the start of the sequence it is possible to fuse other sequence if they follow the Freiburg Biobrick standard 25 and contains an AgeI site. However our part is not entirely built in the Freiburg standard Biobrick 25 since it does not contain an AgeI site.