Difference between revisions of "Part:BBa K1403003"
Line 2: | Line 2: | ||
<partinfo>BBa_K1403003 short</partinfo> | <partinfo>BBa_K1403003 short</partinfo> | ||
− | + | <u> | |
+ | Constitutive promoter | ||
+ | Synthetic RBS | ||
+ | Limonene synthetase (LIMS1) | ||
+ | </u> | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
+ | Transform <i>E. coli</i> for (+)-Limonene biosynthesis. | ||
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Revision as of 10:53, 18 October 2014
Limonene synthase (LIMS1) expression cassette
Constitutive promoter Synthetic RBS Limonene synthetase (LIMS1)
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 82
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 187
Verification
Expected bands at 2kbp for pSB1C3 and 1798bp for the limonene generator