Difference between revisions of "Part:BBa K1341000"
| Line 8: | Line 8: | ||
| − | + | We use this device as a part of a switch device designed for our bio-compass.It can help us screen out the possible path way which include the start point,terminal point, and the particular intermediate nodes(such as the museum(No.5 node)in our story).It has a LTT Promoter,with elements of the tet, lac, they will compose as a device and is responsive to the commonly used inducers IPTG and aTc, producing GFP as an output signal.If this device and tetR(BBa_K1341003)&lacI(BBa_K1341002)successful inserted in the path way by using the typically enzyme site. It will drive expression of green fluorescent protein (GFP). This characteristic will help us to screen out the possible way we want to find.PS: We build a measurement system to test the screen functional about | |
| − | + | ||
| − | + | ||
| − | node)in our story).It has a LTT Promoter,with elements of the tet, lac, they | + | |
| − | will compose as a device and is responsive to the commonly used inducers IPTG | + | |
| − | + | ||
| − | &lacI(BBa_K1341002)successful inserted in the path way by using the typically | + | |
| − | + | ||
| − | + | ||
| − | find.PS: We build a measurement system to test the screen functional about | + | |
this device in our project(BBa_K1341001). | this device in our project(BBa_K1341001). | ||
| Line 34: | Line 25: | ||
| − | The x and y axes form a grid of 36 pairs of inducer concentrations. | + | The x and y axes form a grid of 36 pairs of inducer concentrations. The concentrations tested were 0, 1, 10, 50,100, and 200 ng/mL for aTC and 0, 0.001, 0.002, 0.005,0.010, 0.100, and 1.000mM for IPTG behavior is depicted 6 h after induction. The plotted model values are the means of 1000 independent stochastic kinetic simulations, the source and the reference are in the design page. |
| − | + | ||
| − | aTC and 0, 0.001, 0.002, 0.005,0.010, 0.100, and 1.000mM for IPTG | + | |
| − | + | ||
| − | The plotted model values are the means of 1000 independent stochastic | + | |
| − | + | ||
| Line 65: | Line 51: | ||
| − | Conditions are ±100 ng/mL aTc and ±1mM IPTG, e.g., ± refers to | + | Conditions are ±100 ng/mL aTc and ±1mM IPTG, e.g., ± refers to 100 ng/mL aTc and 1mM IPTG. This panel depicts an LTT promoter |
| − | + | design, where the single lac operator site has been moved upstream of the −35 sequence. In this case, LacI is no longer able to entirely suppress transcription and an intermediate level of induction (leakiness) occurs at conditions of high aTc but no IPTG. All data is obtained 6 h after induction. | |
| − | design, where the single lac operator site has been moved upstream | + | |
| − | + | ||
| − | + | ||
| − | occurs at conditions of high aTc but no IPTG. All data is obtained 6 h | + | |
| − | + | ||
Revision as of 13:36, 12 October 2014
switch device(LTT gfp)for our bio-compass
We use this device as a part of a switch device designed for our bio-compass.It can help us screen out the possible path way which include the start point,terminal point, and the particular intermediate nodes(such as the museum(No.5 node)in our story).It has a LTT Promoter,with elements of the tet, lac, they will compose as a device and is responsive to the commonly used inducers IPTG and aTc, producing GFP as an output signal.If this device and tetR(BBa_K1341003)&lacI(BBa_K1341002)successful inserted in the path way by using the typically enzyme site. It will drive expression of green fluorescent protein (GFP). This characteristic will help us to screen out the possible way we want to find.PS: We build a measurement system to test the screen functional about
this device in our project(BBa_K1341001).
model
The x and y axes form a grid of 36 pairs of inducer concentrations. The concentrations tested were 0, 1, 10, 50,100, and 200 ng/mL for aTC and 0, 0.001, 0.002, 0.005,0.010, 0.100, and 1.000mM for IPTG behavior is depicted 6 h after induction. The plotted model values are the means of 1000 independent stochastic kinetic simulations, the source and the reference are in the design page.
characterizationc
Conditions are ±100 ng/mL aTc and ±1mM IPTG, e.g., ± refers to 100 ng/mL aTc and 1mM IPTG. This panel depicts an LTT promoter
design, where the single lac operator site has been moved upstream of the −35 sequence. In this case, LacI is no longer able to entirely suppress transcription and an intermediate level of induction (leakiness) occurs at conditions of high aTc but no IPTG. All data is obtained 6 h after induction.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 75
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 732