Difference between revisions of "Part:BBa K1341000"
Line 8: Line 8:
  
  
−
We use this device as a part of a switch device designed for our bio-compass. It can help us screen out the possible path way which include the start point, terminal point, and the particular intermediate nodes(such as the museum(No.5 node)in our story).It has a LTT Promoter,with elements of the tet, lac, they will compose as a device and is responsive to the commonly used inducers IPTG and aTc, producing GFP as an output signal.If this device and tetR(BBa_K1341003)&lacI(BBa_K1341002)successful  inserted in the path way by using the typically enzyme site. It will drive expression of green fluorescent protein (GFP). This characteristic will help us to screen out the possible way we want to find.PS: We build a measurement system to test the screen functional about this device in our project(BBa_K1341001).
+
We use this device as a part of a switch device designed for our bio-compass.
 +
It can help us screen out the possible path way which include the start point,
 +
terminal point, and the particular intermediate nodes(such as the museum(No.5  
 +
node)in our story).It has a LTT Promoter,with elements of the tet, lac, they  
 +
will compose as a device and is responsive to the commonly used inducers IPTG
 +
and aTc, producing GFP as an output signal.If this device and tetR(BBa_K1341003)
 +
&lacI(BBa_K1341002)successful  inserted in the path way by using the typically
 +
enzyme site. It will drive expression of green fluorescent protein (GFP).
 +
This characteristic will help us to screen out the possible way we want to  
 +
find.PS: We build a measurement system to test the screen functional about
 +
this device in our project(BBa_K1341001).
  
  
Line 24: Line 34:
  
  
−
The x and y axes form a grid of 36 pairs of inducer concentrations. The concentrations tested were 0, 1, 10, 50,100, and 200 ng/mL for aTC and 0, 0.001, 0.002, 0.005,0.010, 0.100, and 1.000mM for IPTG behavior is depicted 6 h after induction.  
+
The x and y axes form a grid of 36 pairs of inducer concentrations.
−
The plotted model values are the means of 1000 independent stochastic kinetic simulations, the source and the reference are in the design page.
+
The concentrations tested were 0, 1, 10, 50,100, and 200 ng/mL for  
 +
aTC and 0, 0.001, 0.002, 0.005,0.010, 0.100, and 1.000mM for IPTG
 +
behavior is depicted 6 h after induction.  
 +
The plotted model values are the means of 1000 independent stochastic
 +
kinetic simulations, the source and the reference are in the design page.
  
  
Line 51: Line 65:
  
  
−
Conditions are ±100 ng/mL aTc and ±1mM IPTG, e.g., ± refers to 100 ng/mL aTc and 1mM IPTG.  
+
Conditions are ±100 ng/mL aTc and ±1mM IPTG, e.g., ± refers to
−
This panel depicts an LTT promoter design, where the single lac operator site has been moved upstream of the
+
100 ng/mL aTc and 1mM IPTG. This panel depicts an LTT promoter
−
−35 sequence. In this case, LacI is no longer able to entirely suppress transcription and an intermediate level of induction (leakiness) occurs at conditions of high aTc but no IPTG. All data is obtained 6 h after induction.
+
design, where the single lac operator site has been moved upstream
 +
of the −35 sequence. In this case, LacI is no longer able to entirely
 +
suppress transcription and an intermediate level of induction (leakiness)  
 +
occurs at conditions of high aTc but no IPTG. All data is obtained 6 h
 +
after induction.
  
  

Revision as of 13:35, 12 October 2014

switch device(LTT gfp)for our bio-compass

Ahutk0006.png



We use this device as a part of a switch device designed for our bio-compass.
It can help us screen out the possible path way which include the start point,
terminal point, and the particular intermediate nodes(such as the museum(No.5

node)in our story).It has a LTT Promoter,with elements of the tet, lac, they will compose as a device and is responsive to the commonly used inducers IPTG 

and aTc, producing GFP as an output signal.If this device and tetR(BBa_K1341003)

&lacI(BBa_K1341002)successful inserted in the path way by using the typically 

enzyme site. It will drive expression of green fluorescent protein (GFP).
This characteristic will help us to screen out the possible way we want to

find.PS: We build a measurement system to test the screen functional about 

this device in our project(BBa_K1341001).





model

Ahutk0002.png


The x and y axes form a grid of 36 pairs of inducer concentrations. 

The concentrations tested were 0, 1, 10, 50,100, and 200 ng/mL for

aTC and 0, 0.001, 0.002, 0.005,0.010, 0.100, and 1.000mM for IPTG 

behavior is depicted 6 h after induction.

The plotted model values are the means of 1000 independent stochastic 

kinetic simulations, the source and the reference are in the design page.









characterizationc

Ahutk0005.png




Conditions are ±100 ng/mL aTc and ±1mM IPTG, e.g., ± refers to 

100 ng/mL aTc and 1mM IPTG. This panel depicts an LTT promoter
design, where the single lac operator site has been moved upstream
of the −35 sequence. In this case, LacI is no longer able to entirely
suppress transcription and an intermediate level of induction (leakiness)

occurs at conditions of high aTc but no IPTG. All data is obtained 6 h 

after induction.








Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 75
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 732