Difference between revisions of "Part:BBa K1442102"
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Reverse GFP for E.coli (RGE) is a specially designed testing module. It contains GFP codon optimised to work in E.coli in the reverse orientation, followed by a ribosome binding site (RBS)……………. After the RBS is the 3’UTR SLD3 containing a ribozyme (BBa_K1442114) which is an RDRP (BBa_K1442100) initiator.   
 
Reverse GFP for E.coli (RGE) is a specially designed testing module. It contains GFP codon optimised to work in E.coli in the reverse orientation, followed by a ribosome binding site (RBS)……………. After the RBS is the 3’UTR SLD3 containing a ribozyme (BBa_K1442114) which is an RDRP (BBa_K1442100) initiator.   
  
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===Usage ===
 
  
  
 
== Usage ==
 
== Usage ==
  
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The RGE was intended to be used to test the different 3’UTR options which can be cloned in using restriction digest enzymes Afl II and BsiW1.
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K1442102 SequenceAndFeatures</partinfo>
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[[File:RGE.jpg]]
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===Functional Parameters===
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''This graphic generated from Geneious shows the organisation of the RGE testing module.
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<partinfo>BBa_K1442102 parameters</partinfo>
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Revision as of 13:32, 12 October 2014

Reverse GFP for E.coli

RGE

Reverse GFP for E.coli (RGE) is a specially designed testing module. It contains GFP codon optimised to work in E.coli in the reverse orientation, followed by a ribosome binding site (RBS)……………. After the RBS is the 3’UTR SLD3 containing a ribozyme (BBa_K1442114) which is an RDRP (BBa_K1442100) initiator.


Usage

The RGE was intended to be used to test the different 3’UTR options which can be cloned in using restriction digest enzymes Afl II and BsiW1.


RGE.jpg This graphic generated from Geneious shows the organisation of the RGE testing module.