Difference between revisions of "Part:BBa J45200:Design"

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<partinfo>BBa_J45200 short</partinfo>
 
<partinfo>BBa_J45200 short</partinfo>
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===Design Notes===
 
===Design Notes===
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This part is the ATF sequence BBa_45J006 with a single point mutation at base pair 1148 (T -> C). Despite the mutation, the enzyme is still functional (kinetic studies have not been done to determine realitive functionality).
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We will be interested in using AATase I to catalyze the reaction: isoamyl alcohol -> isoamyl acetate. Isoamyl acetate is a volatile ester with a strong banana-like scent. (The related enzyme AATase II, which is encoded by the gene ATF2, performs a similar function...but it is less efficient.)
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According to studies done by [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=12937998&query_hl=16&itool=pubmed_docsum Horton et al.], the addition of 10mM isoamyl alcohol to cultured E. coli expressing the ATF1 gene yields 1.8mM isoamyl acetate after 40 hours.
  
 
===Source===
 
===Source===
  
 
===References===
 
===References===

Revision as of 04:34, 31 October 2006

Banana odor generator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 182
    Illegal BamHI site found at 1509
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 609
    Illegal SapI.rc site found at 1617


Design Notes

We will be interested in using AATase I to catalyze the reaction: isoamyl alcohol -> isoamyl acetate. Isoamyl acetate is a volatile ester with a strong banana-like scent. (The related enzyme AATase II, which is encoded by the gene ATF2, performs a similar function...but it is less efficient.)

According to studies done by [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=12937998&query_hl=16&itool=pubmed_docsum Horton et al.], the addition of 10mM isoamyl alcohol to cultured E. coli expressing the ATF1 gene yields 1.8mM isoamyl acetate after 40 hours.

Source

References