Difference between revisions of "Part:BBa K1537016"
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| − | 2A peptide sequences were found in Picornaviruses to mediate | + | 2A peptide sequences were found in Picornaviruses to mediate "cleavage" between two proteins. We use 2A peptide-linked |
| − | "cleavage" between two proteins. We use 2A peptide-linked | + | multicistronic vectors to express multiple proteins from a single open reading frame (ORF) effectively. To minimize the risk of homologous recombination, it is important to use different 2A peptide sequences if more than two genes are being linked. The 2A peptide system has thus far worked successfully in all eukaryotic systems tested, from mammalian cells, yeast, and plants.(A El et al，2004)[[File:abc.jpg]] |
| − | multicistronic vectors to express multiple proteins from a single open | + | |
| − | reading frame (ORF) effectively.[[File:abc.jpg]] | + | |
| + | == '''GSG linker''' == | ||
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| + | GSG linker is an oligopeptide of “Gly-Ser-Gly” between your protein and 2A peptide to enhance cleavage.( Andrea L et al，2012) | ||
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| + | References | ||
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| + | Abdelhak El Amrani,Abdellah Barakate,Barak M. Askari, Xuejun Li, Alison G. Roberts,Martin D. Ryan, and Claire Halpin.(2004)Coordinate Expression and Independent Subcellular Targeting of Multiple Proteins from a Single Transgene.Plant Physiology.Vol. 135, pp. 16–24 | ||
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| + | Andrea L. Szymczak-Workman, Kate M. Vignali, and Dario A.A. Vignali.(2012)Design and construction of 2A vectors.Cold Spring Harb Protoc :199-204. | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K1537016 SequenceAndFeatures</partinfo> | <partinfo>BBa_K1537016 SequenceAndFeatures</partinfo> | ||
Revision as of 04:33, 12 October 2014
GSG linker+P2A
P2A
2A peptide sequences were found in Picornaviruses to mediate "cleavage" between two proteins. We use 2A peptide-linked
multicistronic vectors to express multiple proteins from a single open reading frame (ORF) effectively. To minimize the risk of homologous recombination, it is important to use different 2A peptide sequences if more than two genes are being linked. The 2A peptide system has thus far worked successfully in all eukaryotic systems tested, from mammalian cells, yeast, and plants.(A El et al，2004)
GSG linker
GSG linker is an oligopeptide of “Gly-Ser-Gly” between your protein and 2A peptide to enhance cleavage.( Andrea L et al，2012)
References
Abdelhak El Amrani,Abdellah Barakate,Barak M. Askari, Xuejun Li, Alison G. Roberts,Martin D. Ryan, and Claire Halpin.(2004)Coordinate Expression and Independent Subcellular Targeting of Multiple Proteins from a Single Transgene.Plant Physiology.Vol. 135, pp. 16–24
Andrea L. Szymczak-Workman, Kate M. Vignali, and Dario A.A. Vignali.(2012)Design and construction of 2A vectors.Cold Spring Harb Protoc :199-204.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]