Difference between revisions of "Part:BBa J34100:Design"
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===Source=== | ===Source=== | ||
− | + | When both the mRNA for the t7RNA polymerase and the tRNA that deactivates the early stop transcription are present, a functional t7RNA can be produced. | |
===References=== | ===References=== |
Revision as of 14:58, 20 October 2006
AND gate, 2x PoPS input, 1x PoPS output
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 15
Illegal EcoRI site found at 2963
Illegal XbaI site found at 27 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 15
Illegal EcoRI site found at 2963 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 15
Illegal EcoRI site found at 2963
Illegal BamHI site found at 9
Illegal XhoI site found at 3048 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 15
Illegal EcoRI site found at 2963
Illegal XbaI site found at 27 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 15
Illegal EcoRI site found at 2963
Illegal XbaI site found at 27
Illegal NgoMIV site found at 1968
Illegal NgoMIV site found at 2087
Illegal NgoMIV site found at 2702
Illegal NgoMIV site found at 2711
Illegal AgeI site found at 2262 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The initial promoters are omitted, so that the light sensing system can accept PoPs from another device, and the promoter on the end produces PoPs from this device
Source
When both the mRNA for the t7RNA polymerase and the tRNA that deactivates the early stop transcription are present, a functional t7RNA can be produced.