Difference between revisions of "Part:BBa K1355003"

Revision as of 21:46, 11 October 2014

Mercury ions accumulator device

The Bioaccumulative Biobrick is composed of our “Essential biobrick” (BBa_K1355001) + a Metallothionein (BBa_K346004) available in the database of the iGEM since 2010 ,as represented below:

In mercury’s presence, MerR protein will bind to it so MerT, MerP and Metal Binding Protein (MBP) proteins are synthesized and bioaccumulation will start! MerP and MerT protein are responsible for mercury’s transport of mercury from the periplasm to cytoplasm, leading it to MBP! The MBP binds to the mercury in the cytoplasm, inactivating it.

Figure 1: Mercury Bacter Hg bioaccumulator (DH5-alpha transformed with BBa_K1355003)

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 988
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 586
Illegal NgoMIV site found at 1160
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI site found at 579

@@ Line 8: / Line 8: @@
 In mercury’s presence, MerR protein will bind to it so MerT, MerP and Metal Binding Protein (MBP) proteins are synthesized and bioaccumulation will start! MerP and MerT protein are responsible for mercury’s transport of mercury from the periplasm to cytoplasm, leading it to MBP! The MBP binds to the mercury in the cytoplasm, inactivating it.
-[[File:MercuryBacterBIoacc.jpg]]
+[[File:MercuryBacterBIOACC.jpg]]
 Figure 1:  Mercury Bacter Hg bioaccumulator (DH5-alpha transformed with BBa_K1355003)