Difference between revisions of "Part:BBa K1497007"
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This composite part to produce naringenin from tyrosine, if put under a promotor. | This composite part to produce naringenin from tyrosine, if put under a promotor. | ||
It consists of 4 genes: | It consists of 4 genes: | ||
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<html> | <html> | ||
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<div align="right"> | <div align="right"> | ||
<table class="MsoTableGrid" | <table class="MsoTableGrid" | ||
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src="https://static.igem.org/mediawiki/parts/4/47/Pathway_I_Gesamtgleichung.png"></p> | src="https://static.igem.org/mediawiki/parts/4/47/Pathway_I_Gesamtgleichung.png"></p> | ||
− | <p class="MsoCaption" align="text-align:justify"><span lang="EN-US"><b>Figure | + | <p class="MsoCaption" align="text-align:justify"><span lang="EN-US"><b>Figure 1</b></span></a><span lang="EN-US"> |
E. coli Top10 with different Naringenin biosensors. Left: On agar plate without naringenin no colour is visible. Middle: On agar plate with 100 µM naringenin colour is visible, except of negative sample <a href="/Part:BBa_K1497019">BBa_K1497019</a> without fluorphor. Right: On agar plate with 100 µM Naringenin under UV light. The fluorescence of GFP, CFP and mKate is visible. <br></span></p> | E. coli Top10 with different Naringenin biosensors. Left: On agar plate without naringenin no colour is visible. Middle: On agar plate with 100 µM naringenin colour is visible, except of negative sample <a href="/Part:BBa_K1497019">BBa_K1497019</a> without fluorphor. Right: On agar plate with 100 µM Naringenin under UV light. The fluorescence of GFP, CFP and mKate is visible. <br></span></p> | ||
</td> | </td> | ||
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</html> | </html> | ||
− | <td style="padding: 0cm 5.4pt; vertical-align: top; width: 236.7pt; height: 214.9pt;"> | + | ===Usage and Biology=== |
− | + | ||
+ | <html> | ||
+ | <div align="right"> | ||
+ | <table class="MsoTableGrid" | ||
+ | style="border: medium none ; border-collapse: collapse; text-align: left; margin-left: auto; margin-right: auto;" | ||
+ | border="0" cellpadding="0" cellspacing="0"> | ||
+ | <tbody> | ||
+ | <tr style="height: 214.9pt;"> | ||
+ | <td style="padding: 0cm 5.4pt; vertical-align: top; width: 236.7pt; height: 214.9pt;"> | ||
+ | blablablablablablbalbabalbal | ||
+ | |||
<li>4-CL <a href="/Part:BBa_K1033001">BBa_K1033001</a></li> | <li>4-CL <a href="/Part:BBa_K1033001">BBa_K1033001</a></li> | ||
<li>TAL <a href="/Part:BBa_K1033000">BBa_K1033000</a></li> | <li>TAL <a href="/Part:BBa_K1033000">BBa_K1033000</a></li> | ||
<li>CHI <a href="/Part:BBa_K1497100">BBa_K1497100</a></li> | <li>CHI <a href="/Part:BBa_K1497100">BBa_K1497100</a></li> | ||
<li>CHS <a href="/Part:BBa_K1497101">BBa_K1497101</a></li> | <li>CHS <a href="/Part:BBa_K1497101">BBa_K1497101</a></li> | ||
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+ | <td | ||
+ | style="padding: 0cm 5.4pt; vertical-align: top; width: 250.7pt; height: 170.9pt;"> | ||
+ | <img style="width: 300px; height: 33,4px;" alt="" | ||
+ | src="https://static.igem.org/mediawiki/parts/7/7d/Naringenin_Operon-_genetic_map.png"></p> | ||
+ | |||
+ | <p class="MsoCaption" align="text-align:justify"><span lang="EN-US"><b>Figure 2</b></span></a><span lang="EN-US"> | ||
+ | E. coli Top10 with different Naringenin biosensors. Left: On agar plate without naringenin no colour is visible. Middle: On agar plate with 100 µM naringenin colour is visible, except of negative sample <a href="/Part:BBa_K1497019">BBa_K1497019</a> without fluorphor. Right: On agar plate with 100 µM Naringenin under UV light. The fluorescence of GFP, CFP and mKate is visible. <br></span></p> | ||
+ | </td> | ||
+ | |||
+ | </tr> | ||
+ | <tbody> | ||
+ | </table> | ||
+ | </div> | ||
+ | </html> | ||
For a strong and stable translation in front of each gene the RBS Bba_B0034 is implemented. | For a strong and stable translation in front of each gene the RBS Bba_B0034 is implemented. | ||
+ | |||
===Functional Parameters=== | ===Functional Parameters=== | ||
<partinfo>BBa_K1497007 parameters</partinfo> | <partinfo>BBa_K1497007 parameters</partinfo> | ||
+ | |||
+ | <html> | ||
+ | <div align="right"> | ||
+ | <table class="MsoTableGrid" | ||
+ | style="border: medium none ; border-collapse: collapse; text-align: left; margin-left: auto; margin-right: auto;" | ||
+ | border="0" cellpadding="0" cellspacing="0"> | ||
+ | <tbody> | ||
+ | <tr style="height: 214.9pt;"> | ||
+ | <td style="padding: 0cm 5.4pt; vertical-align: top; width: 236.7pt; height: 214.9pt;"> | ||
+ | blablablablablablbalbabalbal | ||
+ | |||
+ | <li>4-CL <a href="/Part:BBa_K1033001">BBa_K1033001</a></li> | ||
+ | <li>TAL <a href="/Part:BBa_K1033000">BBa_K1033000</a></li> | ||
+ | <li>CHI <a href="/Part:BBa_K1497100">BBa_K1497100</a></li> | ||
+ | <li>CHS <a href="/Part:BBa_K1497101">BBa_K1497101</a></li> | ||
+ | |||
+ | <td | ||
+ | style="padding: 0cm 5.4pt; vertical-align: top; width: 250.7pt; height: 170.9pt;"> | ||
+ | <img style="width: 300px; height: 33,4px;" alt="" | ||
+ | src="https://static.igem.org/mediawiki/parts/e/e6/Naringeninoperont7coli.png"></p> | ||
+ | |||
+ | <p class="MsoCaption" align="text-align:justify"><span lang="EN-US"><b>Figure 2</b></span></a><span lang="EN-US"> | ||
+ | E. coli Top10 with different Naringenin biosensors. Left: On agar plate without naringenin no colour is visible. Middle: On agar plate with 100 µM naringenin colour is visible, except of negative sample <a href="/Part:BBa_K1497019">BBa_K1497019</a> without fluorphor. Right: On agar plate with 100 µM Naringenin under UV light. The fluorescence of GFP, CFP and mKate is visible. <br></span></p> | ||
+ | </td> | ||
+ | |||
+ | </tr> | ||
+ | <tbody> | ||
+ | </table> | ||
+ | </div> | ||
+ | </html> | ||
+ | |||
+ | <html> | ||
+ | |||
+ | <div align="right"> | ||
+ | <table class="MsoTableGrid" | ||
+ | style="border: medium none ; border-collapse: collapse; text-align: left; margin-left: auto; margin-right: auto;" | ||
+ | border="0" cellpadding="0" cellspacing="0"> | ||
+ | <tbody> | ||
+ | <tr style="height: 214.9pt;"> | ||
+ | |||
+ | |||
+ | <td | ||
+ | style="padding: 0cm 5.4pt; vertical-align: top; width: 250.7pt; height: 170.9pt;"> | ||
+ | <img style="width: 900px; height: 99,4px;" alt="" | ||
+ | src="https://static.igem.org/mediawiki/parts/b/b5/NaringenBALK.png"></p> | ||
+ | |||
+ | <p class="MsoCaption" align="text-align:justify"><span lang="EN-US"><b>Figure 1</b></span></a><span lang="EN-US"> | ||
+ | E. coli Top10 with different Naringenin biosensors. Left: On agar plate without naringenin no colour is visible. Middle: On agar plate with 100 µM naringenin colour is visible, except of negative sample <a href="/Part:BBa_K1497019">BBa_K1497019</a> without fluorphor. Right: On agar plate with 100 µM Naringenin under UV light. The fluorescence of GFP, CFP and mKate is visible. <br></span></p> | ||
+ | </td> | ||
+ | |||
+ | </tr> | ||
+ | <tbody> | ||
+ | </table> | ||
+ | </div> | ||
+ | </html> | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Revision as of 21:01, 11 October 2014
Naringenin producing operon
This composite part to produce naringenin from tyrosine, if put under a promotor. It consists of 4 genes:
Figure 1
E. coli Top10 with different Naringenin biosensors. Left: On agar plate without naringenin no colour is visible. Middle: On agar plate with 100 µM naringenin colour is visible, except of negative sample BBa_K1497019 without fluorphor. Right: On agar plate with 100 µM Naringenin under UV light. The fluorescence of GFP, CFP and mKate is visible. |
Usage and Biology
blablablablablablbalbabalbal
|
Figure 2
E. coli Top10 with different Naringenin biosensors. Left: On agar plate without naringenin no colour is visible. Middle: On agar plate with 100 µM naringenin colour is visible, except of negative sample BBa_K1497019 without fluorphor. Right: On agar plate with 100 µM Naringenin under UV light. The fluorescence of GFP, CFP and mKate is visible. |
For a strong and stable translation in front of each gene the RBS Bba_B0034 is implemented.
Functional Parameters
blablablablablablbalbabalbal
|
Figure 2
E. coli Top10 with different Naringenin biosensors. Left: On agar plate without naringenin no colour is visible. Middle: On agar plate with 100 µM naringenin colour is visible, except of negative sample BBa_K1497019 without fluorphor. Right: On agar plate with 100 µM Naringenin under UV light. The fluorescence of GFP, CFP and mKate is visible. |
Figure 1
E. coli Top10 with different Naringenin biosensors. Left: On agar plate without naringenin no colour is visible. Middle: On agar plate with 100 µM naringenin colour is visible, except of negative sample BBa_K1497019 without fluorphor. Right: On agar plate with 100 µM Naringenin under UV light. The fluorescence of GFP, CFP and mKate is visible. |
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1104
Illegal NheI site found at 4631 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1671
Illegal BglII site found at 4640
Illegal XhoI site found at 3638 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1777
Illegal NgoMIV site found at 2609
Illegal NgoMIV site found at 4611
Illegal NgoMIV site found at 5187
Illegal AgeI site found at 1872
Illegal AgeI site found at 2038 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 3067
Illegal BsaI site found at 4560
Illegal BsaI.rc site found at 1303
Illegal BsaI.rc site found at 4042