Difference between revisions of "Part:BBa K1497007"
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For a strong and stable translation in front of each gene the RBS Bba_B0034 is implemented.  
 
For a strong and stable translation in front of each gene the RBS Bba_B0034 is implemented.  
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===Functional Parameters===
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<partinfo>BBa_K1497007 parameters</partinfo>
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 20:09, 11 October 2014

Naringenin producing operon

This composite part to produce naringenin from tyrosine, if put under a promotor. It consists of 4 genes:




Figure 2 E. coli Top10 with different Naringenin biosensors. Left: On agar plate without naringenin no colour is visible. Middle: On agar plate with 100 µM naringenin colour is visible, except of negative sample BBa_K1497019 without fluorphor. Right: On agar plate with 100 µM Naringenin under UV light. The fluorescence of GFP, CFP and mKate is visible.

<td style="padding: 0cm 5.4pt; vertical-align: top; width: 236.7pt; height: 214.9pt;">


  • 4-CL <a href="/Part:BBa_K1033001">BBa_K1033001</a>
  • TAL <a href="/Part:BBa_K1033000">BBa_K1033000</a>
  • CHI <a href="/Part:BBa_K1497100">BBa_K1497100</a>
  • CHS <a href="/Part:BBa_K1497101">BBa_K1497101</a>

    • </td>


    For a strong and stable translation in front of each gene the RBS Bba_B0034 is implemented.

    Functional Parameters

    Sequence and Features


    Assembly Compatibility:
    • 10
      COMPATIBLE WITH RFC[10]
    • 12
      INCOMPATIBLE WITH RFC[12]
      Illegal NheI site found at 1104
      Illegal NheI site found at 4631
    • 21
      INCOMPATIBLE WITH RFC[21]
      Illegal BglII site found at 1671
      Illegal BglII site found at 4640
      Illegal XhoI site found at 3638
    • 23
      COMPATIBLE WITH RFC[23]
    • 25
      INCOMPATIBLE WITH RFC[25]
      Illegal NgoMIV site found at 1777
      Illegal NgoMIV site found at 2609
      Illegal NgoMIV site found at 4611
      Illegal NgoMIV site found at 5187
      Illegal AgeI site found at 1872
      Illegal AgeI site found at 2038
    • 1000
      INCOMPATIBLE WITH RFC[1000]
      Illegal BsaI site found at 3067
      Illegal BsaI site found at 4560
      Illegal BsaI.rc site found at 1303
      Illegal BsaI.rc site found at 4042