Difference between revisions of "Part:pSB1A7:Design"
(→Design Notes) |
(→Source) |
||
| Line 15: | Line 15: | ||
===Source=== | ===Source=== | ||
| − | Based upon pSB1A3 | + | Based upon pSB1A3. Includes two double terminators assembled from DNA oligos based upon the sequence of [https://parts.igem.org/wiki/index.php/Part:BBa_B0015 BBa_B0015]. |
===References=== | ===References=== | ||
Revision as of 17:49, 19 October 2006
No part name specified with partinfo tag.
No part name specified with partinfo tag.
Design Notes
Purpose
Read-through transcription is extremely problematic when the usefulness of a device depends upon an off state. This plasmid was constructed to insulate BioBrick parts from read-through transcription coming from the backbone.
Double Forward and Backwards Terminator Assembly
The double terminators were assembled from smaller overlapping ssDNA oligos. The outer-most EcoRI and PstI sites were mutated while the inner-most BioBrick cut sites were restored.
Source
Based upon pSB1A3. Includes two double terminators assembled from DNA oligos based upon the sequence of BBa_B0015.