Difference between revisions of "Part:BBa K1412614"

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BBa_K1412614: pBAD- RBS (1.0)-<i>CheZ</i>-TT
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'''BBa_K1412614: pBAD- RBS (1.0)-<i>CheZ</i>-TT'''
 
        
 
        
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This part consists of a <i>[http://en.wikipedia.org/wiki/Chemotaxis CheZ] </i> gene which can express CheZ protein deciding <i>E.coli</i> whether tumbling or swimming straight. In this light, we can characterize the efficiency of promoter by just replacing different promoters before a <i>CheZ</i> gene. Then we can characterize the efficiency of promoter via measuring the migration distance positively associated with the expression strength of CheZ protein.
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This part consists of <i>[http://en.wikipedia.org/wiki/Chemotaxis CheZ] </i> gene which can express CheZ protein make <i>E.coli</i> tumbling or swimming straight. In this light, we can characterize the efficiency of promoter by replacing different promoters before <i>CheZ</i> gene. Then we can characterize the efficiency of promoter via measuring the migration distance positively associated with the expression strength of CheZ protein.
  
  
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When we want to characterize the efficiency of promoter, we usually link the promoter with GFP, then characterize the efficiency of promoter by measuring the fluorescence intensity of GFP. In our part, you need just link RBS (1.0) after a pBAD promoter and before a <i>CheZ</i> gene, ended with a TT terminator (pBAD-RBS (1.0)-<i>CheZ</i>-TT). Then transfer this gene circuit into <i>E.coli</i> (<i>CheZ</i> knocked out), coat plates, and culture on semi-solid medium to measure the migration diameter of <i>E.coli</i>.
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When we want to characterize the efficiency of promoter, we usually connect the promoter with GFP, then measuring the fluorescence intensity of GFP. In our part, you just need connect RBS (1.0) after pBAD promoter and before <i>CheZ</i> gene, ended with doiuble terminator (pBAD-RBS (1.0)-<i>CheZ</i>-TT). Then transfer this gene circuit into <i>E.coli</i> (<i>CheZ</i> knocked out), coat plates, and culture on semi-solid medium to measure the migration diameter of <i>E.coli</i>.
  
  
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<bbpart>BBa_K1412829</bbpart>: pLac-RBS(0.3)-CheZ-TT Characterize efficiency of RBS with chemotaxis.
 
<bbpart>BBa_K1412829</bbpart>: pLac-RBS(0.3)-CheZ-TT Characterize efficiency of RBS with chemotaxis.
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==='''Notes'''===
 
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===='''Source'''====
 
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<bbpart>BBa_K206000</bbpart>
 
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<bbpart>BBa_B0034</bbpart>
 
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<bbpart>BBa_K629003</bbpart>
 
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<bbpart>BBa_B0015</bbpart>
 
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<I><B>More information, click here: [http://2014.igem.org/Team:XMU-China# XMU-China]
 
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ameter display  
 
ameter display  

Revision as of 17:53, 14 October 2014

Characterize the efficiency of promoter(BBa_K206000) with chemotaxis

BBa_K1412614: pBAD- RBS (1.0)-CheZ-TT

This part consists of [http://en.wikipedia.org/wiki/Chemotaxis CheZ] gene which can express CheZ protein make E.coli tumbling or swimming straight. In this light, we can characterize the efficiency of promoter by replacing different promoters before CheZ gene. Then we can characterize the efficiency of promoter via measuring the migration distance positively associated with the expression strength of CheZ protein.


Characterization process design.png


Usage

When we want to characterize the efficiency of promoter, we usually connect the promoter with GFP, then measuring the fluorescence intensity of GFP. In our part, you just need connect RBS (1.0) after pBAD promoter and before CheZ gene, ended with doiuble terminator (pBAD-RBS (1.0)-CheZ-TT). Then transfer this gene circuit into E.coli (CheZ knocked out), coat plates, and culture on semi-solid medium to measure the migration diameter of E.coli.


Relevant parts

BBa_K1412000: pLac-RBS(1.0)-CheZ-TT CheZ generator under pLac promoter.

BBa_K1412005: RBS(1.0)-CheZ-TT.

BBa_K1412006: RBS(0.01)-CheZ-TT.

BBa_K1412007: RBS(0.3)-CheZ-TT.

BBa_K1412014: pTetR-RBS(1.0)-CheZ-TT Characterize the efficiency of promoters with chemotaxis.

BBa_K1412801: pLac-RBS(0.01)-CheZ-TT Characterize the efficiency of RBS with chemotaxis.

BBa_K1412829: pLac-RBS(0.3)-CheZ-TT Characterize efficiency of RBS with chemotaxis.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Reference

More information, click here: [http://2014.igem.org/Team:XMU-China# XMU-China]