Revision as of 09:07, 11 October 2014

Basic part of biobrick BBa_K1412001 and BBa_K1412033

What it is

Basic part of biobrick K1412001, lacking of promoter BBa_K206000(pTET).

What it does

Through ligating this part with different promoters such as pBAD, pTET, pCONS and transform the plasmid into E.coli(CL-1), we can endow the bacteria the ability of responding to different inducers and inhibitors. On the basis of this mechanism, we could take the E.coli(CL-1) under our command to form different pattern which is mathematically meaning.

How to use it

To form ellipse, we ligate this part with promoter pCONS and transform the plasmid into E.coli(CL-1), then we cultivate the bacteria on the plate with M63 medium controlling the concentration of IPTG and chloromycetin. Doting IPTG on the plate is the most critical procedure on the whole course. By tracking and measuring the chemotaxis diameter of the bacteria on the plate, we get a series of experimental data, More detail in Experimental Data.

To form hyperbolic curve and parabola, we use promoter pBAD which is specifically recognized by L-Arabinose(pTET which is specially recognized by anhydrotetracycline). Then we cultivate the bacteria on the plate with M63 medium controlling the concentration of IPTG, L-Arabinose (anhydrotetracycline) and chloromycetin. After the M63 medium dries up, we dot the IPTG and L-Arabinose (anhydrotetracycline) on the plate to form the mathematically meaningful pattern. By tracking and measuring the chemotaxis diameter of the bacteria on the plate, we get a series of experimental data, more detail in Experimental Data.

Experimental Data

With K20600(pBAD) as an inhibit promoter

The characterization results of part BBa_K1412001

With R0040(pTET) as an inhibit promoter

The characterization results of part BBa_K1412033

protocol

1.Transformation

2.Extract plasmids

3.Digestion

4.DNA gel electrophoresis

5.Gel Extraction

6.Ligation

7.Transformation

8.Extract plasmids

9.Digestion

10.DNA gel electrophoresis

11.Transform the plasmid into CL-1

Sequence and Features