Difference between revisions of "Part:BBa K1481005"

Latest revision as of 20:11, 10 October 2014

Strobable rhamnose AND temperature operated transcriptor SR-latch

This device converts result of the AND operation on rhamnose concentration and temperature signals into ampified superfolder GFP expression. It comprises of a rhamnose-inducible promoter pRha with the U10-U RNA thermometer, Bxb1 recombinase, the radB module and the CDS of the reporter protein. R4 integrase recognition sites allow signal strobing by reporter CDS cleavage using BBa_K1481006.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 355
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 125
Illegal BamHI site found at 629
Illegal XhoI site found at 716
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 1924
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 1944
Illegal BsaI.rc site found at 1463
Illegal BsaI.rc site found at 1827
Illegal BsaI.rc site found at 2101
Illegal SapI.rc site found at 2193

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 <partinfo>BBa_K1481005 short</partinfo>
-This device converts result of the AND operation on rhamnose concentration and temperature signals into ampified superfolder GFP  expression. It comprises of a rhamnose-inducible promoter pRha with the U10-U RNA thermometer, Bxb1 recombinase, the radB module and the CDS of the reporter protein. R4 integrase recognition sites allow signal strobing by reporter CDS cleavage.
+This device converts result of the AND operation on rhamnose concentration and temperature signals into ampified superfolder GFP  expression. It comprises of a rhamnose-inducible promoter pRha with the U10-U RNA thermometer, Bxb1 recombinase, the radB module and the CDS of the reporter protein. R4 integrase recognition sites allow signal strobing by reporter CDS cleavage using [https://parts.igem.org/Part:BBa_K1481006 BBa_K1481006].
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